Phillips G D, Lu D Y, Mitashov V I, Carlson B M
Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor 48109.
Am J Anat. 1987 Dec;180(4):365-72. doi: 10.1002/aja.1001800407.
The objective of this study was to determine how long myogenic cells can survive in the central ischemic zone of early free muscle grafts in the rat. The study was conducted on free grafts of a large (rectus femoris) and a small (extensor digitorum longus) muscle. At times ranging from zero hr to five days post-grafting, the central zones were isolated, minced, and implanted under the back skin of mice. After five days the minces were removed and examined histologically for the presence of rat myotubes, which should form only in minces that contain viable myogenic cells. The results show that myogenic cells survive two to four hr in the ischemic centers of the large rectus femoris grafts; after longer post-grafting intervals, rat myotubes did not arise in central zone minces. In grafts of small muscles, myotubes consistently appeared in central zone minces. Since the formerly ischemic central areas of rectus muscle grafts are ultimately replaced by regenerating muscle fibers, we conclude that these regenerating muscle fibers are derived from precursor cells located outside of the ischemic zone.
本研究的目的是确定生肌细胞在大鼠早期游离肌肉移植物的中央缺血区能够存活多长时间。该研究针对一块大肌肉（股直肌）和一块小肌肉（趾长伸肌）的游离移植物展开。在移植后从零小时到五天的不同时间点，分离出中央区域，切碎后植入小鼠背部皮肤下。五天后取出切碎组织，进行组织学检查，以确定是否存在大鼠肌管，因为肌管仅会在含有存活生肌细胞的切碎组织中形成。结果显示，生肌细胞在大型股直肌移植物的缺血中心存活两到四个小时；在移植后的更长间隔时间后，中央区域切碎组织中未出现大鼠肌管。在小肌肉移植物中，中央区域切碎组织中始终会出现肌管。由于股直肌移植物先前的缺血中央区域最终会被再生肌纤维所取代，我们得出结论，这些再生肌纤维源自位于缺血区之外的前体细胞。
