Rushton J L, Davies I, Horan M A, Mahon M, Williams R
School of Biological Sciences, University of Manchester, UK.
J Anat. 1997 Apr;190 ( Pt 3)(Pt 3):417-22. doi: 10.1046/j.1469-7580.1997.19030417.x.
The crush model of injury in skeletal muscle is widely used in the investigation of tissue degeneration and regeneration. Previously, such trauma has been induced by using forceps to crush the muscle, commonly applying sufficient pressure to bring the mid-arms of the forceps together. This report introduces a reliable electromechanical device designed to generate reproducible focal lesions in skeletal muscle of mice. The tibialis anterior was crushed in 17 young adult mice. Two days after injury, the muscles were examined microscopically. By morphometric analysis, it was determined that the volumes of the lesions produced were similar (mean 0.499 mm3 +/- 0.098, range 0.278 - 0.601 mm3), and that the full extent of the damaged muscle was easily distinguished and readily quantifiable. This will allow a more precise comparison in future investigations into regenerative differences between age groups, satellite cell activation and the inflammatory response.
骨骼肌挤压损伤模型在组织退变和再生研究中被广泛应用。此前，此类创伤是通过用镊子挤压肌肉来诱导的，通常施加足够的压力使镊子的中臂合拢。本报告介绍了一种可靠的机电装置，旨在在小鼠骨骼肌中产生可重复的局灶性损伤。对17只年轻成年小鼠的胫前肌进行了挤压。损伤两天后，对肌肉进行显微镜检查。通过形态计量分析，确定所产生损伤的体积相似（平均0.499立方毫米±0.098，范围0.278 - 0.601立方毫米），并且受损肌肉的完整范围易于区分且易于量化。这将使未来在不同年龄组之间的再生差异、卫星细胞激活和炎症反应的研究中能够进行更精确的比较。
