Clin Lab. 2021 Jul 1;67(7). doi: 10.7754/Clin.Lab.2020.201029.
Pneumococcal capsular-type identification is essential for monitoring the epidemiology of pneumococcal infections and for establishing the effectiveness of pneumococcal vaccines. The objective of this work was to compare the accuracy of four methods of Streptococcus pneumoniae capsular typing.
A prospective blind study was carried out at Donostia University Hospital (northern Spain) to determine the capsular types of 50 pneumococcal clinical isolates using four techniques: a) S. PneumoStripTM: a reverse-hybridization strip-based commercial assay that detects 76 pneumococcal serotypes: 42 individually and 34 in pairs. b) FAF-mPCR: a single-step multiplex-PCR (polymerase chain reaction) assay combined with fragment analysis using automated fluorescent capillary electrophoresis, which can differentiate 92 serotypes in a single tube: 31 individually, 28 in pairs, and 33 in groups of 3 to 5 serotypes. c) PCRSeqTyping: which enables the detection of 91 serotypes after sequencing the regions of the cpsB gene in two steps: 59 directly and the remaining 32 serotypes in a second step. d) The Quellung reaction.
The S. PneumoStripTM, FAF-mPCR and PCRSeqTyping identified the serotypes of all the 50 clinical isolates. With the Quellung reaction 46/50 (92%) isolates were correctly serotyped. The quickest technique was the S. PneumoStripTM, followed by the single-step multiplex PCR assay and PCRSeqTyping. The Quellung reaction was the slowest technique.
The S. PneumoStripTM, PCRSeqTyping, and FAF-mPCR were very accurate techniques for pneumococcal serotyping, with S. PneumoStripTM obtaining results more rapidly. The combination of any of these S. pneumoniae molecular typing techniques and the Quellung reaction as confirmation reference method is a highly precise and fast strategy for the serotyping of high number of pneumococcal clinical isolates.
肺炎球菌荚膜型鉴定对于监测肺炎球菌感染的流行病学和评估肺炎球菌疫苗的有效性至关重要。本研究的目的是比较四种肺炎球菌荚膜分型方法的准确性。
在西班牙北部的多诺斯蒂亚大学医院进行了一项前瞻性、盲法研究，使用四种技术来确定 50 株肺炎球菌临床分离株的荚膜型：a）S. PneumoStripTM：一种基于反向杂交的商业检测试剂盒，可检测 76 种肺炎球菌血清型：42 种单独检测，34 种成对检测。b）FAF-mPCR：一种单管一步法多重 PCR（聚合酶链反应）检测方法，结合自动化荧光毛细管电泳片段分析，可在单个管中区分 92 种血清型：31 种单独检测，28 种成对检测，33 种 3 至 5 种血清型成组检测。c）PCRSeqTyping：通过两步法检测 cpsB 基因区域的序列，可检测 91 种血清型：59 种直接检测，其余 32 种血清型在第二步检测。d）Quellung 反应。
S. PneumoStripTM、FAF-mPCR 和 PCRSeqTyping 鉴定了 50 株临床分离株的血清型。Quellung 反应正确鉴定了 46/50（92%）株分离株。最快的技术是 S. PneumoStripTM，其次是单步多重 PCR 检测和 PCRSeqTyping。Quellung 反应是最慢的技术。
S. PneumoStripTM、PCRSeqTyping 和 FAF-mPCR 是非常准确的肺炎球菌血清分型技术，S. PneumoStripTM 获得结果更快。将这些 S. pneumoniae 分子分型技术中的任何一种与 Quellung 反应相结合作为确认参考方法，是对大量肺炎球菌临床分离株进行血清分型的高度精确和快速策略。
