Northrop D B, Duggleby R G
School of Pharmacy, University of Wisconsin, Madison 53706.
Anal Biochem. 1987 Sep;165(2):362-4. doi: 10.1016/0003-2697(87)90282-x.
A method for obtaining highly purified NADH in a dry, solid, and stable form is described. The method involves improvements of the ion-exchange and reversed-phase chromatographic procedures of C. J. Newton and S. M. Faynor, and D. B. Northrop (Anal. Biochem., 1983, 132, 50-53). The necessary time to prepare pure NADH has been reduced to a few hours. The final product, obtained by drying the nucleotide from absolute ethanol, shows no detectable decomposition either during the drying procedure or during storage under nitrogen gas at -20 degrees C for several months. Using dry product prepared from fixed volumes of ethanolic solution, standardized solutions of known amounts of the highly purified and stored NADH can be obtained in a few seconds.
本文描述了一种以干燥、固体且稳定的形式获得高纯度烟酰胺腺嘌呤二核苷酸（NADH）的方法。该方法改进了C. J. 牛顿、S. M. 费诺以及D. B. 诺思罗普（《分析生物化学》，1983年，第132卷，第50 - 53页）的离子交换和反相色谱程序。制备纯NADH所需的时间已缩短至数小时。通过从无水乙醇中干燥核苷酸得到的最终产物，在干燥过程中以及在 - 20℃氮气气氛下储存数月期间均未检测到分解。使用从固定体积乙醇溶液制备的干燥产物，可在几秒钟内获得已知量的高纯度储存NADH的标准化溶液。
