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氧化型和还原型烟酰胺腺嘌呤二核苷酸的高效离子交换分离

High-performance ion-exchange separation of oxidized and reduced nicotinamide adenine dinucleotides.

作者信息

Orr G A, Blanchard J S

出版信息

Anal Biochem. 1984 Oct;142(1):232-4. doi: 10.1016/0003-2697(84)90544-x.

DOI:10.1016/0003-2697(84)90544-x
PMID:6517317
Abstract

High-performance anion-exchange chromatography of oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) on a Pharmacia Mono Q anion-exchange column is reported. Microgram quantities of all four nucleotides can be separated at pH 7.7 in approximately 20 min. For preparative purposes, greater than 7 mg of NADH can be purified in a single injection, and the peak fractions have an A260 of greater than 80 OD units with an A260/A340 ratio of 2.25.

摘要

报道了在Pharmacia Mono Q阴离子交换柱上对氧化型和还原型烟酰胺腺嘌呤二核苷酸（NAD）及烟酰胺腺嘌呤二核苷酸磷酸（NADP）进行高效阴离子交换色谱分析。在pH 7.7条件下，约20分钟内可分离微克量的所有四种核苷酸。用于制备时，单次进样可纯化超过7毫克的NADH，峰馏分的A260大于80 OD单位，A260/A340比值为2.25。

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