人血小板中腺嘌呤和吡啶核苷酸的碱性提取及反相高效液相色谱法
Alkaline extraction and reverse-phase high-performance liquid chromatography of adenine and pyridine nucleotides in human platelets.
作者信息
Leoncini G, Buzzi E, Maresca M, Mazzei M, Balbi A
机构信息
Institute of Biological Chemistry, University of Genoa, Italy.
出版信息
Anal Biochem. 1987 Sep;165(2):379-83. doi: 10.1016/0003-2697(87)90285-5.
The levels of adenine (ATP, ADP, AMP) and pyridine (NAD, NADH) nucleotides in human platelets have been measured by a simple and reproducible method. A rapid alkaline extraction allows a complete recovery of the compounds concerned. The metabolic ATP and ADP in the cytosolic fraction, the amount released upon thrombin stimulation, and the ADP bound to F-actin have also been evaluated. Analysis was performed by reverse-phase, isocratic high-performance liquid chromatography on a 5-microns Lichrosorb RP-18 column with uv detection at 254 nm.
已通过一种简单且可重复的方法测量了人血小板中腺嘌呤（ATP、ADP、AMP）和吡啶（NAD、NADH）核苷酸的水平。快速碱性提取可实现相关化合物的完全回收。还评估了胞质部分中的代谢ATP和ADP、凝血酶刺激后释放的量以及与F-肌动蛋白结合的ADP。分析是在一根5微米的Lichrosorb RP-18柱上通过反相等度高效液相色谱法进行的，在254纳米处进行紫外检测。
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