人红细胞中腺嘌呤和吡啶核苷酸的同时提取及反相高效液相色谱测定

Simultaneous extraction and reverse-phase high-performance liquid chromatographic determination of adenine and pyridine nucleotides in human red blood cells.

作者信息

Stocchi V, Cucchiarini L, Magnani M, Chiarantini L, Palma P, Crescentini G

出版信息

Anal Biochem. 1985 Apr;146(1):118-24. doi: 10.1016/0003-2697(85)90405-1.

Abstract

A simple and rapid method for the determination of ATP, ADP, AMP, NADP+, NAD+, NADPH, and NADH in human erythrocytes is described. A single-step extraction procedure employing alkaline medium and CF 50A Amicon ultrafiltration membranes allows a simultaneous and total recovery of the compounds of interest. Analysis is performed by reverse-phase high-performance liquid chromatography on a 5-micron Supelcosil LC-18 column and uv detection. Extraction and analysis require about 30 min. Levels of adenine and pyridine nucleotides in normal adults are also presented.

摘要

描述了一种用于测定人红细胞中ATP、ADP、AMP、NADP⁺、NAD⁺、NADPH和NADH的简单快速方法。采用碱性介质和CF 50A密理博超滤膜的单步提取程序可同时完全回收目标化合物。通过在5微米的Supelcosil LC-18柱上进行反相高效液相色谱和紫外检测进行分析。提取和分析大约需要30分钟。还给出了正常成年人中腺嘌呤和吡啶核苷酸的水平。