Bowie E P, Ishikawa H, Shiino M, Rennels E G
J Histochem Cytochem. 1978 Feb;26(2):94-7. doi: 10.1177/26.2.342596.
The 2A8 clone, a normal diploid rat anterior pituitary cell strain, was investigated by immunocytochemistry to determine the cell types into which the clonal cells differentiated in vivo and in vitro. The in vivo study was carried out by injecting the 2A8 clone preparation either into the hypothalamic region or under the kidney capsule. After thirty days the implants were removed and studied by immunocytochemistry. In vitro, many prolactin cells and a few growth hormone cells were found. In vivo, however, prolactin, growth hormone, ACTH and TSH cells and gonadotrophs were identified. We concluded that the 2A8 clone was multipotential. Since the gonadotrophs of the implants made in the hypothalamic region were larger and more plentiful than those in the kidney implants, and since gonadotrophs were lacking in the in vitro system, it appeared that the hypophysiotrophic environment was the most conducive to gonadotrophic differentiation and maintenance, and that the factor or factors necessary for cyto-differentiation were apparently present in the general circulation of the rat but absent in the growth medium of our culture cells.
2A8克隆系一种正常二倍体大鼠垂体前叶细胞株，通过免疫细胞化学方法对其进行研究，以确定该克隆细胞在体内和体外分化而成的细胞类型。体内研究是将2A8克隆体制剂注射到下丘脑区域或肾被膜下进行的。30天后取出植入物，通过免疫细胞化学进行研究。在体外发现了许多催乳素细胞和少量生长激素细胞。然而，在体内鉴定出了催乳素、生长激素、促肾上腺皮质激素、促甲状腺激素细胞和促性腺激素细胞。我们得出结论，2A8克隆具有多能性。由于在下丘脑区域植入物中的促性腺激素细胞比肾植入物中的更大且更丰富，并且由于体外系统中缺乏促性腺激素细胞，因此看来垂体营养环境最有利于促性腺激素细胞的分化和维持，而且细胞分化所需的一种或多种因子显然存在于大鼠的全身循环中，但不存在于我们培养细胞的生长培养基中。
