Shiino M, Ishikawa H, Rennels E G
Cell Tissue Res. 1977 Jul 19;181(4):473-85. doi: 10.1007/BF00221769.
A clonal strain of anterior pituitary cells was derived from Rathke's pouch of the rat. These cells were shown to secrete ACTH, growth hormone and prolactin but no glycoprotein hormones, when grown in vitro. Cells from the 2A8 clone were implanted for one month under kidney capsules or into hypothalami of hypophysectomized female rats. Under the kidney capsule, prominent prolactin cells and poorly developed cells of other types were differentiated as seen in usual pituitary grafts. In hypophysiotrophic areas of the hypothalamus, the grafts were cytodifferentiated into various types of anterior pituitary cells with rich vascularization. These cells had the ultrastructural features indicative of hormone secretion. Increases in body and ovarian weights reflected the secretion of somatotrophic and gonadotrophic hormones. The results obtained indicate that implants of 2A8 clonal cells may differentiate into all types of anterior pituitary cells under the influence of hypothalamic hormones or perhaps some unknown factors present in the general systemic circulation of the rat.
一种垂体前叶细胞的克隆株源自大鼠的拉特克囊。当这些细胞在体外培养时，已显示它们能分泌促肾上腺皮质激素、生长激素和催乳素，但不分泌糖蛋白激素。将来自2A8克隆的细胞植入垂体切除的雌性大鼠的肾被膜下或下丘脑内一个月。在肾被膜下，可见到突出的催乳素细胞以及发育不良的其他类型细胞，这与通常的垂体移植所见相同。在下丘脑的促垂体区，移植的细胞分化为各种类型的垂体前叶细胞，血管丰富。这些细胞具有表明激素分泌的超微结构特征。体重和卵巢重量的增加反映了生长激素和促性腺激素的分泌。所得结果表明，在大鼠全身循环中存在的下丘脑激素或可能某些未知因素的影响下，2A8克隆细胞植入物可能分化为所有类型的垂体前叶细胞。
