Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487-0203, USA.
J Mater Chem B. 2021 Aug 21;9(31):6103-6115. doi: 10.1039/d1tb00963j. Epub 2021 Jul 14.
Hyaluronic acid (HA) is a natural polysaccharide and a key component of the extracellular matrix (ECM) in many tissues. Therefore, HA-based biomaterials are extensively utilized to create three dimensional ECM mimics to study cell behaviors in vitro. Specifically, derivatives of HA have been commonly used to fabricate hydrogels with controllable properties. In this review, we discuss the various chemistries employed to fabricate HA-based hydrogels as a tunable matrix to mimic the cancer microenvironment and subsequently study cancer cell behaviors in vitro. These include Michael-addition reactions, photo-crosslinking, carbodiimide chemistry, and Diels-Alder chemistry. The utility of these HA-based hydrogels to examine cancer cell behaviors such as proliferation, migration, and invasion in vitro in various types of cancer are highlighted. Overall, such hydrogels provide a biomimetic material-based platform to probe cell-matrix interactions in cancer cells in vitro and study the mechanisms associated with cancer progression.
透明质酸（HA）是一种天然多糖，也是许多组织细胞外基质（ECM）的关键组成部分。因此，基于 HA 的生物材料被广泛用于构建三维 ECM 模拟物，以研究细胞在体外的行为。具体来说，HA 的衍生物通常被用于制备具有可控性能的水凝胶。在这篇综述中，我们讨论了用于制备基于 HA 的水凝胶的各种化学方法，这些水凝胶作为可调节的基质来模拟癌症微环境，随后在体外研究癌症细胞的行为。这些方法包括迈克尔加成反应、光交联、碳二亚胺化学和 Diels-Alder 化学。强调了这些基于 HA 的水凝胶在各种类型的癌症中用于研究体外癌细胞增殖、迁移和侵袭等行为的用途。总的来说，这些水凝胶为体外研究癌症细胞中细胞-基质相互作用和研究与癌症进展相关的机制提供了基于仿生材料的平台。
