Nuño-Soriano Ana, Arias-Hidalgo Carlota, Montalvillo Enrique, Góngora Rafael, Hernández Ángela-Patricia, Juanes-Velasco Pablo, Fuentes Manuel
Translational and Clinical Research Program, Cytometry Service, NUCLEUS and Department of Medicine, Cancer Center Research (IBMSS-CSIC), University of Salamanca, 37008 Salamanca, Spain.
Proteomics Unit-IBSAL, Institute of Biomedical Research of Salamanca, University of Salamanca (IBSAL-USAL), 37007 Salamanca, Spain.
Nanomaterials (Basel). 2025 Jul 3;15(13):1032. doi: 10.3390/nano15131032.
Biocompatibility remains a central issue for introducing biomaterials and nanomedicines into the clinic, requiring safety, functionality, toxicity prevention, and the control of foreign body reactions. Therefore, it is necessary to evaluate multiple biomaterial parameters and molecular interactions affecting cell functions, like apoptosis, adhesion, proliferation, or spreading, as well as intracellular signals and cellular microenvironment status. Although conventional well-established in vitro techniques are helpful at the first stages of bio and nanomaterials development, high-throughput techniques expand the screening and designing possibilities. This review presents high-throughput functional proteomics approaches, focused on protein microarrays and mass spectrometry techniques, for the evaluation of biocompatibility in the new era of biomedicine.
生物相容性仍然是将生物材料和纳米药物引入临床的核心问题，这需要安全性、功能性、毒性预防以及对异物反应的控制。因此，有必要评估影响细胞功能的多个生物材料参数和分子相互作用，如细胞凋亡、粘附、增殖或铺展，以及细胞内信号和细胞微环境状态。尽管传统的成熟体外技术在生物和纳米材料开发的最初阶段很有帮助，但高通量技术扩展了筛选和设计的可能性。本综述介绍了高通量功能蛋白质组学方法，重点是蛋白质微阵列和质谱技术，用于评估生物医学新时代的生物相容性。
