植物乳杆菌胞外多糖生物合成与胃肠道耐受性的相关性。

Correlation between exopolysaccharide biosynthesis and gastrointestinal tolerance of Lactiplantibacillus plantarum.

机构信息

College of Food Science and Technology, Yangzhou University, Yangzhou, China.

Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou, China.

出版信息

J Appl Microbiol. 2022 Jan;132(1):584-591. doi: 10.1111/jam.15213. Epub 2021 Jul 21.

DOI:10.1111/jam.15213

PMID:34261198

Abstract

AIM

This study aimed to investigate the correlation between the level of exopolysaccharide (EPS) biosynthesis and gastrointestinal tolerance of 12 Lactiplantibacillus plantarum strains.

METHODS AND RESULTS

In this study, the EPS production and survival rate of 12 strains of L. plantarum under gastrointestinal stress were determined. Results showed that the EPS biosynthesis level of L. plantarum in semi-defined medium ranged from 9.84 to 26.05 mg/L. The survival rates of all strains in simulated gastric juice at pH 3.0 ranged from 43.52% to 112.73%. Among them, eight strains were higher than 90%, while only one strain was lower than 50%. The survival rates of all strains in simulated intestinal juice ranged from 50.36% to 125.39%, among which eight strains were higher than 80%. The survival rates of all strains under 0.1% bile salt stress ranged from 3.39% to 109.34%, among which four strains were higher than 80% and three strains were lower than 60%. Besides, the survival rates of all strains under 0.5% bile salt stress ranged from 0.42% to 95.34%. The results indicated that the 12 L. plantarum strains had good tolerance to simulated gastric juice at pH 3.0, simulated intestinal juice and 0.1% bile salt. Notably, it was observed that the survival rates of L. plantarum strains under simulated gastric juice at pH 3.0 and simulated intestinal juice were significantly positively correlated with EPS biosynthesis (p < 0.01).

CONCLUSION

The yield of EPS of L. plantarum was related to simulated gastric juice and simulated intestinal juice environment.

SIGNIFICANCE AND IMPACT OF STUDY

It was speculated that the production of EPS may be one of the strategies for L. plantarum to adapt to the part of gastrointestinal environment. In the future, we could analyse the protection mechanism of EPS from the gene level.

摘要

目的

本研究旨在探讨 12 株植物乳杆菌（Lactiplantibacillus plantarum）胞外多糖（EPS）生物合成水平与胃肠道耐受性之间的相关性。

方法与结果

本研究测定了 12 株 L. plantarum 在胃肠道应激下的 EPS 产生和存活率。结果表明，L. plantarum 在半合成培养基中的 EPS 生物合成水平为 9.84 至 26.05mg/L。所有菌株在 pH 值为 3.0 的模拟胃液中的存活率为 43.52%至 112.73%。其中，8 株菌高于 90%，而仅有 1 株菌低于 50%。所有菌株在模拟肠液中的存活率为 50.36%至 125.39%，其中 8 株菌高于 80%。所有菌株在 0.1%胆汁盐胁迫下的存活率为 3.39%至 109.34%，其中 4 株菌高于 80%，3 株菌低于 60%。此外，所有菌株在 0.5%胆汁盐胁迫下的存活率为 0.42%至 95.34%。结果表明，12 株 L. plantarum 对 pH 值为 3.0 的模拟胃液、模拟肠液和 0.1%胆汁盐具有良好的耐受性。值得注意的是，观察到 L. plantarum 菌株在 pH 值为 3.0 的模拟胃液和模拟肠液中的存活率与 EPS 生物合成呈显著正相关（p<0.01）。