College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, 163316, China.
Daqing Branch of Heilongjiang Academy of Sciences, Daqing, 163319, China.
Sci Rep. 2021 Jul 14;11(1):14476. doi: 10.1038/s41598-021-93820-6.
Uniconazole (S-(+)-uniconazole), a plant growth retardant, exerts key roles in modulating growth and development and increasing abiotic stress tolerance in plants. However, the underlying mechanisms by which uniconazole regulates drought response remain largely unknown. Here, the effects of exogenous uniconazole on drought tolerance in hemp were studied via physiological and transcriptome analyses of the drought-sensitive industrial hemp cultivar Hanma No. 2 grown under drought stress. Exogenous uniconazole treatment increased hemp tolerance to drought-induced damage by enhancing chlorophyll content and photosynthesis capacity, regulating activities of enzymes involved in carbon and nitrogen metabolism, and altering endogenous hormone levels. Expression of genes associated with porphyrin and chlorophyll metabolism, photosynthesis-antenna proteins, photosynthesis, starch and sucrose metabolism, nitrogen metabolism, and plant hormone signal transduction were significantly regulated by uniconazole compared with that by control (distilled water) under drought stress. Numerous genes were differentially expressed to increase chlorophyll content, enhance photosynthesis, regulate carbon-nitrogen metabolism-related enzyme activities, and alter endogenous hormone levels. Thus, uniconazole regulated physiological and molecular characteristics of photosynthesis, carbon-nitrogen metabolism, and plant hormone signal transduction to enhance drought resistance in industrial hemp.
烯效唑（S-(+)-烯效唑），一种植物生长延缓剂，在调节植物生长发育和提高非生物胁迫耐受性方面发挥着关键作用。然而，烯效唑调节干旱响应的潜在机制在很大程度上仍不清楚。本研究通过对干旱敏感型工业大麻品种汉麻 2 号在干旱胁迫下的生理和转录组分析，研究了外源烯效唑对大麻耐旱性的影响。外源烯效唑通过提高叶绿素含量和光合作用能力、调节与碳氮代谢相关的酶活性以及改变内源激素水平来增强大麻对干旱诱导损伤的耐受性。与对照（蒸馏水）相比，干旱胁迫下，与卟啉和叶绿素代谢、光合作用天线蛋白、光合作用、淀粉和蔗糖代谢、氮代谢和植物激素信号转导相关的基因表达明显受到烯效唑的调节。大量基因差异表达，以增加叶绿素含量、增强光合作用、调节碳氮代谢相关酶活性以及改变内源激素水平。因此，烯效唑通过调节光合作用、碳氮代谢和植物激素信号转导的生理和分子特征来增强工业大麻的耐旱性。
