School of Pharmacy, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.
Department of Pharmacy, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, People's Republic of China.
Drug Des Devel Ther. 2021 Jul 6;15:2885-2897. doi: 10.2147/DDDT.S310263. eCollection 2021.
The polysaccharide of (wall.) Lindl. (ARPS) is one of its important active ingredients. Hepatoprotective effects of ARPS on rat liver injury induced by CCl were studied.
ARPS was extracted using the ultrasonic method and successfully purified by high-speed counter-current chromatography (HSCCC) with a two-phase aqueous system composed of 12.5% PEG 1000-20% KHPO:KHPO (1:1). The HSCCC conditions were optimized, and the structure of ARPS was characterized. The hepatoprotective effects of ARPS against CCl-induced chronic hepatic injury in SD rats were evaluated.
The results showed that ARPS was a water-soluble polysaccharide with a molecular weight of 28,518 Da. It was composed of mannose, ribose, glucose, and arabian sugar; its monosaccharide molar ratio was glucose:ribose:arabinose:mannose = 54.24:13.20:1.09:1.00. The purity of ARPS was determined by HPLC to be 96.93%. The intervention effects of ARPS on CCl-induced hepatic damage model in rats showed that ARPS could effectively reduce the activity of alanine amino transferase and aspartate amino transferase, decrease the content of malondialdehyde and nitric oxide synthesis, and increase the content of glutathione. Pathology revealed that liver plate order, liver cell degeneration, and edema were improved; inflammatory cell infiltration was not observed after ARPS intervention.
ARPS had the function of antioxidant for protecting CCl-induced injured liver, and the mechanisms were related to anti-lipid peroxidation, which could eliminate oxygen-free radicals and protect liver cells from attacks by free radicals.
（Wall.）Lindl.（ARPS）的多糖是其重要的活性成分之一。本研究探讨了 ARPS 对 CCl 诱导的大鼠肝损伤的肝保护作用。
采用超声法提取 ARPS，并用高速逆流色谱（HSCCC）结合 12.5%PEG1000-20%KHPO：KHPO（1：1）两相水相体系成功对其进行了纯化。优化了 HSCCC 条件，并对 ARPS 的结构进行了表征。评价了 ARPS 对 SD 大鼠 CCl 诱导的慢性肝损伤的保护作用。
结果表明，ARPS 是一种水溶性多糖，分子量为 28518Da，由甘露糖、核糖、葡萄糖和阿拉伯糖组成；其单糖摩尔比为葡萄糖：核糖：阿拉伯糖：甘露糖=54.24:13.20:1.09:1.00。HPLC 法测定 ARPS 的纯度为 96.93%。ARPS 对 CCl 诱导的大鼠肝损伤模型的干预作用表明，ARPS 能有效降低丙氨酸氨基转移酶和天冬氨酸氨基转移酶的活性，降低丙二醛和一氧化氮合酶的含量，增加谷胱甘肽的含量。病理学显示，肝板排列整齐，肝细胞变性、水肿改善；经 ARPS 干预后未见炎症细胞浸润。
ARPS 具有抗氧化功能，可保护 CCl 诱导的损伤肝脏，其机制与抗脂质过氧化有关，可消除氧自由基，保护肝细胞免受自由基攻击。
