新鲜马奶中抗氧化肽的分离、纯化及特性研究
Isolation, purification, and characterization of antioxidant peptides from fresh mare's milk.
作者信息
Waili Yizaitiguli, Gahafu Yiming, Aobulitalifu Alimijiang, Chang Zhanying, Xie Xiangyun, Kawuli Gulibahaer
机构信息
Department of Pharmaceutics and Physical Chemistry College of Pharmacy, Xinjiang Medical University Urumqi China.
Department of Natural Medicine College of Pharmacy, Xinjiang Medical University Urumqi China.
出版信息
Food Sci Nutr. 2021 May 11;9(7):4018-4027. doi: 10.1002/fsn3.2292. eCollection 2021 Jul.
In this study, the whey protein of fresh mare's milk was used as raw material. The antioxidant peptide liquid XMNDT was extracted from fresh mare's milk solution and purified. The peptide had a molecular weight of 1594.89 kDa and was mainly composed of VAPFPQPVVPYPQR. Antioxidant peptide XMNDT could inhibit the proliferation of A549 lung cancer cells in the G1 phase, accelerate cell apoptosis, increase the activity of SOD and the amount of GSH, and reduce the secretion of MDA. It also exhibited certain antioxidant capacity and free radical scavenging. These data can provide a basis for research on new antioxidant properties by reducing the inflammation caused by aging.
在本研究中，以新鲜马乳的乳清蛋白为原料。从新鲜马乳溶液中提取并纯化了抗氧化肽液XMNDT。该肽的分子量为1594.89 kDa，主要由VAPFPQPVVPYPQR组成。抗氧化肽XMNDT可抑制A549肺癌细胞在G1期的增殖，加速细胞凋亡，增加超氧化物歧化酶（SOD）的活性和谷胱甘肽（GSH）的含量，并减少丙二醛（MDA）的分泌。它还表现出一定的抗氧化能力和自由基清除能力。这些数据可为通过减少衰老引起的炎症来研究新的抗氧化特性提供依据。
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