Department of Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, H.P, India.
Department of Soil Science and Water Management, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan, India.
World J Microbiol Biotechnol. 2021 Jul 15;37(8):135. doi: 10.1007/s11274-021-03101-7.
The present study aimed to isolate and identify root endophytic bacteria with multifunctional plant growth promoting (PGP) traits from medicinal plant Rosmarinus officinalis grown in the North-Western Himalayas. A total of 42 strains were isolated, exhibiting variable degrees of PGP traits, including phosphate solubilization (10-375 µg/mL), indole-3-acetic acid (6-66 µg/mL), siderophore (32.37%-301.48% SU) production and antifungal activity in terms of percent growth inhibition (% GI) against Fusarium oxysporum (44.44%-77.77% GI), Fusarium graminearum (48.88%-71.42% GI) and Rhizoctonia solani (44.44%-77.7% GI). The 16S rDNA sequencing results showed lineage of these strains to 15 genera viz., Aneurinibacillus, Bacillus, Beijerinckia, Cedecea, Ensifer, Enterobacter, Kosakonia, Lactobacillus, Lysobacter, Oxynema, Pseudomonas, Pantoea, Paenibacillus, Pseudoxanthomonas and Serratia. Out of 42 strains, 11 potential strains were selected for in vivo growth studies of R. officinalis. The results showed that the inoculation of Bacillus subtilis KU21, Pseudomonas aeruginosa SI12, and Cedecea lapagei KU14 significantly increased the physical growth parameters of plant over uninoculated control viz., number of lateral of branches (43.95%-46.39%), stem height (29.04%-38.57%), root length (32.31%-37.14%), shoot (34.76%-40.91%) and root biomass (62.89%-70.70%). Physiological characteristics such as total chlorophyll (30.41%-30.96%), phenol (14.43%-24.55%) and carotenoids (34.26%-39.87%) content, also showed a relative increase as compared to uninoculated control; furthermore, the macronutrients (NPK) contents of the plant as well as soil also showed an increase. The developed module may be recommended for sustainable production of R. officinalis in the North-Western Himalayan region without hampering the soil health and fertility.
本研究旨在从生长在西北喜马拉雅山的药用植物迷迭香中分离和鉴定具有多功能植物促生长 (PGP) 特性的根内生细菌。共分离出 42 株菌株，它们表现出不同程度的 PGP 特性，包括磷酸盐溶解（10-375μg/mL）、吲哚-3-乙酸（6-66μg/mL）、铁载体（32.37%-301.48%SU）的产生和抗真菌活性，以对尖孢镰刀菌（44.44%-77.77%GI）、禾谷镰刀菌（48.88%-71.42%GI）和立枯丝核菌（44.44%-77.7%GI）的生长抑制百分比（%GI）表示。16S rDNA 测序结果表明，这些菌株的谱系属于 15 个属，即 Aneurinibacillus、Bacillus、Beijerinckia、Cedecea、Ensifer、Enterobacter、Kosakonia、Lactobacillus、Lysobacter、Oxynema、Pseudomonas、Pantoea、Paenibacillus、Pseudoxanthomonas 和 Serratia。在 42 株菌株中，选择了 11 株具有潜在应用价值的菌株进行迷迭香的体内生长研究。结果表明，枯草芽孢杆菌 KU21、铜绿假单胞菌 SI12 和拉氏亚种 KU14 的接种显著增加了植物的物理生长参数，比未接种对照增加了侧枝数（43.95%-46.39%）、茎高（29.04%-38.57%）、根长（32.31%-37.14%）、茎（34.76%-40.91%）和根生物量（62.89%-70.70%）。与未接种对照相比，总叶绿素（30.41%-30.96%）、酚（14.43%-24.55%）和类胡萝卜素（34.26%-39.87%）的含量等生理特性也有所增加；此外，植物以及土壤中的大量营养元素（NPK）含量也有所增加。该模块可以推荐用于在不损害土壤健康和肥力的情况下在西北喜马拉雅地区可持续生产迷迭香。
