Opt Lett. 2021 Jul 15;46(14):3456-3459. doi: 10.1364/OL.424726.
Stimulated Raman scattering (SRS) microscopy for biomedical analysis can provide a molecular localization map to infer pathological tissue changes. Compared to spontaneous Raman, SRS achieves much faster imaging speeds at reduced spectral coverage. By targeting spectral features in the information dense fingerprint region, SRS allows fast and reliable imaging. We present time-encoded (TICO) SRS microscopy of unstained head-and-neck biopsies in the fingerprint region with molecular contrast. We combine a Fourier-domain mode-locked (FDML) laser with a master oscillator power amplifier (MOPA) to cover Raman transitions from 1500-1800. Both lasers are fiber-based and electronically programmable making this fingerprint TICO system robust and reliable. The results of our TICO approach were cross-checked with a spontaneous Raman micro-spectrometer and show good agreement, paving the way toward clinical applications.
受激拉曼散射（SRS）显微镜可用于生物医学分析，提供分子定位图以推断病理组织变化。与自发拉曼相比，SRS 在降低光谱覆盖范围的情况下实现了更快的成像速度。通过针对信息密集的指纹区域中的光谱特征，SRS 可以实现快速可靠的成像。我们在指纹区域对未经染色的头颈部活检进行了时编码（TICO）SRS 显微镜检查，具有分子对比度。我们结合了基于傅里叶域锁模（FDML）的激光器和主振荡器功率放大器（MOPA），以覆盖从 1500-1800 的 Raman 跃迁。两种激光器均基于光纤且可通过电子编程，这使得该指纹 TICO 系统具有强大的可靠性。我们的 TICO 方法的结果与自发拉曼微谱仪进行了交叉检查，结果吻合良好，为临床应用铺平了道路。
