Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 4 Stigbojlen, DK-1870 Frederiksberg C, Denmark.
Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Laenggass-Strasse 122, CH-3001 Bern, Switzerland.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004881.
[] and the unpublished Bisgaard taxon 35 are associated with respiratory and urogenital tract infections in dogs. A total of 21 strains including the type strain of [] were included in the investigation. Strains of [] and taxon 35 formed a monophyletic group demonstrating at least 97.8 and 96.5% similarities within the group based upon 16S rRNA and gene sequence comparisons, respectively. was the most closely related species to [] and taxon 35 with 96.1 % 16S rRNA gene sequence similarity which is slightly higher than the 95 % separating most genera of the family . However, the conserved protein sequence phylogeny documented a unique position of [] with only 81 % identity to the most closely related species, genomospecies 1 of the genus which is lower than the 85 % separating most genera of the family . The conserved protein sequence identity to , the type species of the genus, was 77%, demonstrating that [] is not properly classified as a member of the genus . On the basis of the phylogenetic comparisons, the taxa [] and taxon 35 are proposed to be included with a novel genus with one species, which is reclassified from [] . Phenotypic characters obtained with isolates genetically approved to represent were in accordance with those of the members of the family and the novel genus can be separated from most of the existing genera by a positive catalase reaction, lack of V-factor requirement for growth, lack of haemolysis of blood agar and negative Voges-Proskauer and urease tests. The novel genus cannot be separated by biochemical and physiological characteristics alone from the genera , , and . However, MALDI-TOF mass spectroscopy and also RpoB amino acid signatures allowed a clear separation from these taxa, supporting the existence of a novel genus. The DNA G+C content is 37.0-37.8 mol% for the genus, based on the whole genomic sequences. The type strain of is CCUG 3714 (=ATCC 19416=NCTC 1659) isolated in 1901 from the prepuce of a dog in Germany.
[] 和未发表的比斯加德分类群 35 与狗的呼吸道和泌尿生殖道感染有关。共有 21 株包括[]的模式株被纳入研究。[]和分类群 35 的菌株形成一个单系群，基于 16S rRNA 和基因序列比较，在组内显示至少 97.8%和 96.5%的相似性。与[]和分类群 35 最密切相关的物种是[]，16S rRNA 基因序列相似度为 96.1%，略高于将该科大多数属分开的 95%。然而，保守蛋白序列系统发育记录了[]的独特位置，与最密切相关的种，即属 1 型基因组种的相似度仅为 81%，低于将该科大多数属分开的 85%。与该属的模式种，即[]的保守蛋白序列同一性为 77%，表明[]不能正确分类为该属的成员。基于系统发育比较，提议将分类群[]和分类群 35 包括在一个新属中，即属，该属有一个种，即从[]重新分类。通过遗传上证实代表的分离株获得的表型特征与科的成员和新属一致，并且可以通过阳性过氧化氢酶反应、生长不需要 V 因子、血琼脂无溶血和阴性 Voges-Proskauer 和脲酶试验将新属与大多数现有属区分开来。新属不能仅通过生化和生理特征与属、、和分开。然而，MALDI-TOF 质谱和 RpoB 氨基酸特征也允许与这些分类群清楚地区分，支持新属的存在。根据全基因组序列，属的 DNA G+C 含量为 37.0-37.8mol%。的模式株 CCUG 3714(=ATCC 19416=NCTC 1659)于 1901 年从德国一只狗的包皮中分离。
