Adhikary Sadhana, Bisgaard Magne, Nicklas Werner, Christensen Henrik
Department of Veterinary Animal Sciences, University of Copenhagen, 4 Stigbøjlen, DK-1870 Frederiksberg C, Denmark.
Professor emeritus, Horsevænget 40, Viby Sjælland, Denmark.
Int J Syst Evol Microbiol. 2018 Feb;68(2):643-650. doi: 10.1099/ijsem.0.002558. Epub 2018 Jan 5.
A total of 29 strains mainly from guinea pigs were investigated by a polyphasic approach that included previously published data. The strains were classified as Bisgaard taxa 5 and 7 by comparison of phenotypic characteristics and the strains showed typical cultural characteristics for members of family Pasteurellaceae and the strains formed two monophyletic groups based on 16S rRNA gene sequence comparison. Partial rpoB sequence analysis as well as published data on DNA-DNA hybridization showed high genotypic relationships within both groups. A new genus with one species, Caviibacterium pharyngocola gen. nov., sp. nov., is proposed to accommodate members of taxon 5 of Bisgaard, whereas members of taxon 7 are proposed as Conservatibacter flavescens gen. nov., sp. nov. The two genera are clearly separated by phenotype from each other and from existing genera of the family Pasteurellaceae. The type strain of Caviibacterium pharyngocola is 7.3 (=CCUG 16493=DSM 105478) and the type strain of Conservatibacter flavescens is 7.4 (=CCUG 24852=DSM 105479=HIM 794-7), both were isolated from the pharynx of guinea pigs.
采用多相分类方法对总共29株主要来自豚鼠的菌株进行了研究，该方法包括先前发表的数据。通过表型特征比较，这些菌株被归类为比斯加德分类单元5和7，并且这些菌株显示出巴斯德氏菌科成员的典型培养特征，基于16S rRNA基因序列比较，这些菌株形成了两个单系群。部分rpoB序列分析以及DNA-DNA杂交的已发表数据显示两组内均存在高度的基因型相关性。提出了一个新属及一个新种，即咽腔豚鼠杆菌（Caviibacterium pharyngocola gen. nov., sp. nov.），以容纳比斯加德分类单元5的成员，而分类单元7的成员被提议为淡黄保守杆菌（Conservatibacter flavescens gen. nov., sp. nov.）。这两个属在表型上彼此以及与巴斯德氏菌科的现有属明显区分开来。咽腔豚鼠杆菌的模式菌株是7.3（=CCUG 16493=DSM 105478），淡黄保守杆菌的模式菌株是7.4（=CCUG 24852=DSM 105479=HIM 79)-7），两者均从豚鼠的咽部分离得到。
