College of Pharmacy, School of Medicine, Hangzhou Normal University, Hangzhou 311121, PR China.
Hangzhou Yuhang District Center for Disease Control and Prevention, PR China.
Food Chem. 2021 Dec 15;365:130545. doi: 10.1016/j.foodchem.2021.130545. Epub 2021 Jul 6.
In this study, mangosteen peel based activated carbon was prepared and first applied as adsorbent in matrix solid-phase dispersion (MSPD) for simultaneously extraction of flavonoids from Dendrobium huoshanense prior to their separation and determination by ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS). The MSPD-UHPLC-Q-TOF/MS method was validated exhaustively. Good linearities (r ≥ 0.9929) were obtained for all target analytes. The limits of detection was in the range of 0.00387-0.159 μg/g. Satisfactory recoveries of six target compounds were between 80.02 and 99.49% and 85.32-99.86% for the low and high spiked level, respectively. Furthermore, relative to other common sorbent, the prepared mangosteen peel based activated carbon was less expensive and more environmentally-friendly. Consequently, the proposed method was a simple, efficient, low-cost, eco-friendly, time-saving and sensitive approach that could be successfully applied to the extraction and determination of flavonoids compounds in complex matrix.
在这项研究中，制备了基于山竹果皮的活性炭，并首次将其作为吸附剂应用于基质固相分散（MSPD）中，用于从霍山石斛中同时提取黄酮类化合物，然后通过超高效液相色谱-四极杆飞行时间串联质谱（UHPLC-Q-TOF/MS）进行分离和测定。对 MSPD-UHPLC-Q-TOF/MS 方法进行了全面验证。所有目标分析物均获得了良好的线性关系（r≥0.9929）。检测限范围为 0.00387-0.159μg/g。对于低和高加标水平，六种目标化合物的回收率分别在 80.02%至 99.49%和 85.32%至 99.86%之间。此外，与其他常见的吸附剂相比，制备的基于山竹果皮的活性炭价格更低廉，对环境更友好。因此，该方法简单、高效、低成本、环保、省时且灵敏，可成功应用于复杂基质中黄酮类化合物的提取和测定。
