Yue Zi-Xuan, Cao Jun
College of pharmacy, Hangzhou Normal University, Hangzhou 311121, China.
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China.
Foods. 2022 Jan 1;11(1):111. doi: 10.3390/foods11010111.
In this study, matrix solid phase dispersion (MSPD) microextraction combined with supercritical fluid chromatography-ion mobility quadrupole time-of-flight mass spectrometry (SFC/IM-QTOF-MS) was used to analyze the multipolar compounds in plant tea. The parameters of stationary phase, mobile phase, make-up solution, temperature, and back pressure were optimized. The target analytes were gradient eluted in 8 min by supercritical CO on a Zorbax RX-SIL column. Collisional Cross Section (CCS) values for single and multiple fields were measured. A series of validation studies were carried out under the optimal conditions, and the linear relationship and reproducibility were good. The limits of detection were 1.4 (Scoparone (1))70 (Naringenin (4)) ng/mL, and the limits of quantification were 4.7 (Scoparone (1))241 (Naringenin (4)) ng/mL. The recoveries of most compounds ranged from 60.7% to 127%. As a consequence, the proposed method was used for the separation and quantitative analysis of active ingredients in caulis dendrobii.
在本研究中，采用基质固相分散（MSPD）微萃取结合超临界流体色谱 - 离子淌度四极杆飞行时间质谱（SFC/IM-QTOF-MS）对植物茶中的多极性化合物进行分析。对固定相、流动相、补充液、温度和背压等参数进行了优化。目标分析物在Zorbax RX-SIL柱上用超临界CO₂在8分钟内进行梯度洗脱。测量了单场和多场的碰撞截面积（CCS）值。在最佳条件下进行了一系列验证研究，线性关系和重现性良好。检测限为1.4（滨蒿内酯（1））70（柚皮素（4））ng/mL，定量限为4.7（滨蒿内酯（1））241（柚皮素（4））ng/mL。大多数化合物的回收率在60.7%至127%之间。因此，该方法用于石斛茎中活性成分的分离和定量分析。
