Zajicek J, Pearlman J D, Merickel M B, Ayers C R, Brookeman J R, Brown M F
Department of Chemistry, University of Virginia, Charlottesville 22901.
Biochem Biophys Res Commun. 1987 Dec 16;149(2):437-42. doi: 10.1016/0006-291x(87)90386-x.
Well-resolved proton (1H) NMR spectra of solid human arterial plaque can be acquired. Studies have been carried out of human fatty plaque obtained postmortem (ex vivo), the total lipids extracted from human atheroma, and a model mixture of cholesteryl esters whose lipid composition resembles that of human atheroma. In each case, well-resolved 1H NMR spectra were obtained at body temperature (37 degrees C), with little or no underlying broad signal. Such sharp 1H NMR spectra are typical of isotropic fluids, whereas solid and liquid-crystalline materials give rise to much broader spectral lines. The results suggest the sharp 1H NMR spectra of human atheromatous lesions at body temperature are due largely to the presence of intracellular and extracellular droplets of cholesteryl esters in the isotropic liquid phase. These findings provide a necessary basis for use of 1H NMR techniques to image quantitatively the lipid constituents of human atheroma in vivo, and to study their chemical and physical properties.
可以获取分辨率良好的人体动脉粥样硬化斑块的质子（1H）核磁共振谱。已对死后获得的人体脂肪斑块（离体）、从人体动脉粥样硬化中提取的总脂质以及脂质组成类似于人体动脉粥样硬化的胆固醇酯模型混合物进行了研究。在每种情况下，均在体温（37摄氏度）下获得了分辨率良好的1H核磁共振谱，几乎没有或完全没有潜在的宽信号。这种尖锐的1H核磁共振谱是各向同性流体的典型特征，而固体和液晶材料会产生宽得多的谱线。结果表明，人体动脉粥样硬化病变在体温下的尖锐1H核磁共振谱主要是由于各向同性液相中细胞内和细胞外胆固醇酯液滴的存在。这些发现为使用1H核磁共振技术在体内对人体动脉粥样硬化的脂质成分进行定量成像以及研究其化学和物理性质提供了必要的基础。
