Vlasov V V, Knorre D G, Kutiavin I V, Mamaev S V, Podust L M
Bioorg Khim. 1987 Sep;13(9):1221-9.
Modification of a single-stranded DNA fragment 303 nucleotides long with addressed reagents d(pTGACCCTCTTCCC) A greater than CHRCl (I), d(pACCCTCTTCCC) A greater than CHRCl (II), d(CCTCTTCCC) A greater than CHRCl (III) and d(TCTTCCC) A greater than CHRCl (IV) complementary to the sequence 261-274 has been studied. It was shown that not only G258 residue, located near to the above sequence, but also G179 residue is modified. The latter can be explained by the vicinity of G179 and the alkylating group in the three-dimensional structure of the complex. Some modification of fragment 19-24 was observed due to non-complementary binding of the reagent. Association constants of the reagents (I)-(IV) with 261-274 sequence of the fragment were calculated using the dependence of the modification extent of G258 and G179 on the reagent concentration. The constants at 25 and 35 degrees C were found to be 260 and 31 (I), 0,5 and 2 (II), 0,46 and 0,13 (III), 0,0020 and 0,0023 (IV) microM-1.
用与261 - 274序列互补的靶向试剂d(pTGACCCTCTTCCC) A大于CHRCl (I)、d(pACCCTCTTCCC) A大于CHRCl (II)、d(CCTCTTCCC) A大于CHRCl (III)和d(TCTTCCC) A大于CHRCl (IV)对一条303个核苷酸长的单链DNA片段进行修饰的研究。结果表明，不仅位于上述序列附近的G258残基被修饰，而且G179残基也被修饰。后者可以通过复合物三维结构中G179与烷基化基团的接近程度来解释。由于试剂的非互补结合，观察到片段19 - 24有一些修饰。利用G258和G179的修饰程度对试剂浓度的依赖性，计算了试剂(I)-(IV)与片段261 - 274序列的缔合常数。发现在25℃和35℃时，常数分别为260和31 (I)、0.5和2 (II)、0.46和0.13 (III)、0.0020和0.0023 (IV) μM-1。
