近交系小鼠肝脏乙醇脱氢酶表达的遗传控制

Genetic control of liver alcohol dehydrogenase expression in inbred mice.

作者信息

Patterson L S, Zhang K, Edenberg H J, Bosron W F

机构信息

Department of Medicine, Indiana University School of Medicine, Indianapolis 46223.

出版信息

Alcohol Alcohol Suppl. 1987;1:157-9.

PMID:3426672

Abstract

The expression of the mouse Adh-1 gene which encodes the ADH-AA isoenzyme of liver alcohol dehydrogenase is under genetic control. The content of alcohol dehydrogenase in the C57BL inbred strain of mice is about twice that in DBA mice. In order to examine the mechanism for control of liver alcohol dehydrogenase expression in mice, we have isolated liver RNA from high and low enzyme activity strains and quantified the content of ADH mRNA with the ADH-A cDNA that was recently cloned in our laboratory. The content of ADH-A mRNA in liver correlates well with enzyme activity in the mouse strains; hence, the expression of Adh-1 appears to be under transcriptional control. A restriction fragment polymorphism was found in mouse liver DNA which correlates with the content of ADH-AA in the high and low ADH activity strains.

摘要

编码肝脏乙醇脱氢酶ADH - AA同工酶的小鼠Adh - 1基因的表达受遗传控制。C57BL近交系小鼠体内乙醇脱氢酶的含量约为DBA小鼠的两倍。为了研究小鼠肝脏乙醇脱氢酶表达的调控机制，我们从高酶活性和低酶活性品系中分离出肝脏RNA，并使用我们实验室最近克隆的ADH - A cDNA对ADH mRNA的含量进行了定量。肝脏中ADH - A mRNA的含量与小鼠品系中的酶活性密切相关；因此，Adh - 1的表达似乎受转录控制。在小鼠肝脏DNA中发现了一种限制性片段多态性，它与高ADH活性和低ADH活性品系中ADH - AA的含量相关。