Jeyasingham M D, Pratt O E, Shaw G K, Thomson A D
Department of Neuropathology, Institute of Psychiatry, London, U.K.
Alcohol Alcohol. 1987;22(4):359-65.
Erythrocyte transketolase activation by thiamin diphosphate has been studied in alcoholic patients on admission and after treatment, which included vitamin therapy. The high proportion of the patients who showed an abnormal activation of transketolase, not only by 0.3 mM thiamin diphosphate but also further activation by increasing the thiamin diphosphate to 3 mM, was reduced considerably after treatment. A few patients, however, still showed continuing abnormalities even after treatment. Further study of the enzyme activation in vitro confirmed the presence of an enzyme variant, abnormal both in the ease with which the thiamin diphosphate could be removed and in requiring a high concentration of thiamin diphosphate for activation. Since the modified transketolase activation test appears not only to monitor the effectiveness of thiamin therapy but also independently to warn of persisting enzyme abnormalities, it could prove to be of general use in alcohol detoxification units.
对酒精性患者入院时及接受包括维生素治疗在内的治疗后，通过硫胺素二磷酸对红细胞转酮醇酶激活情况进行了研究。不仅在0.3 mM硫胺素二磷酸作用下，而且在将硫胺素二磷酸浓度增加至3 mM时仍表现出转酮醇酶异常激活的患者比例，在治疗后大幅降低。然而，仍有少数患者即使在治疗后仍表现出持续异常。对酶体外激活的进一步研究证实存在一种酶变体，其在去除硫胺素二磷酸的难易程度以及激活所需的高浓度硫胺素二磷酸方面均异常。由于改良的转酮醇酶激活试验似乎不仅能监测硫胺素治疗的效果，还能独立提示持续存在的酶异常，因此它可能在酒精解毒单位中具有普遍用途。
