Probe the relationship between and in and mouse.

Restless legs syndrome (RLS) is a neurological disorder characterized by an urge to move and uncomfortable sensations. Genetic studies have identified polymorphisms in up to 19 risk loci, including and Rodents deficient in either homolog show RLS-like phenotypes. However, whether and interact is unclear. Here, with , we observed that the hyperactive egg-laying behavior caused by loss of homolog was counteracted by knockdown of homolog. This was further investigated in mutant mice with or both knocked out. The double knockout mice showed an earlier onset of the motor deficit in the wheel running test but did not have increased sensitivity to the heat stimuli as observed in single KOs. Meis1 protein level was not influenced by deficiency, and transcription was not affected by haploinsufficiency. Our results demonstrate that and do not regulate each other.