Borle A B, Studer R
J Membr Biol. 1978 Jan 12;38(1-2):51-72. doi: 10.1007/BF01875162.
The effects of calcium ionophores on cellular calcium metabolism were studied in cultured kidney cells, in cells freshly isolated from rat kidney, and in liver and kidney slices. In isolated cells, these ionophores decreased the total cellular Ca content and the mitochondrial Ca. 45Ca efflux from prelabelled cells was also stimulated even in the absence of extracellular Ca. In slices, the ionphore A23187 increased the total slice Ca and the uptake of 45Ca. However, the mitochondria isolated from these slices treated with the ionophore had a lower total Ca and a depressed relative radioactivity. These results suggest that the increased cytosolic Ca produced by Ca ionophores may be due to mobilization of intracellular Ca stores rather than to a net shift of Ca from the extracellular fluids to the cell.
在培养的肾细胞、从大鼠肾脏新鲜分离的细胞以及肝脏和肾脏切片中研究了钙离子载体对细胞钙代谢的影响。在分离的细胞中，这些离子载体降低了细胞总钙含量和线粒体钙含量。即使在没有细胞外钙的情况下，预先标记细胞的45Ca外流也受到刺激。在切片中，离子载体A23187增加了切片总钙含量和45Ca的摄取。然而，从用该离子载体处理的这些切片中分离的线粒体总钙含量较低，相对放射性降低。这些结果表明，钙离子载体产生的细胞溶质钙增加可能是由于细胞内钙储存的动员，而不是钙从细胞外液向细胞的净转移。
