Reinhart P H, van de Pol E, Taylor W M, Bygrave F L
Biochem J. 1984 Mar 1;218(2):415-20. doi: 10.1042/bj2180415.
The effect of systematically altering the isolation conditions on the total calcium content of mitochondria isolated from perfused rat liver was examined. We showed that, under most isolation conditions, significant redistributions of mitochondrial calcium occurred resulting in up to 5-fold changes of the total calcium content. Mitochondrial Ca2+ flux inhibitors such as Ruthenium Red and nupercaine were only partially effective in inhibiting such redistributions. We present evidence indicating that the total calcium content of rat liver mitochondria in situ may approximate 2 nmol X (mg of protein)-1.
研究了系统性改变分离条件对从灌注大鼠肝脏分离出的线粒体总钙含量的影响。我们发现，在大多数分离条件下，线粒体钙会发生显著的重新分布，导致总钙含量变化高达5倍。线粒体Ca2+通量抑制剂，如钌红和纽白卡因，在抑制这种重新分布方面仅部分有效。我们提供的证据表明，原位大鼠肝脏线粒体的总钙含量可能约为2 nmol X（毫克蛋白）-1。
