National Research Council Canada, Saskatoon, SK, Canada.
Nutrien Ag Solutions, Saskatoon, SK, Canada.
Methods Mol Biol. 2021;2289:263-270. doi: 10.1007/978-1-0716-1331-3_17.
The production of doubled haploid (DH) plants from microspores is an important technique used in plant breeding and basic research. DH technology is a rapid method for developing homozygous lines, which can be used to accelerate crop improvement programs. Haploidy technology can also be used in mutagenesis, transformation, and basic research such as genomic, biochemical, and physiological studies. There is no general protocol that will result in the production of DH in all species, as differences occur among species and among genotypes within a species in terms of embryogenic response. Here we describe methodology for developing doubled haploids in cow cockle (Saponaria vaccaria L.).
从小孢子生产双单倍体（DH）植株是植物育种和基础研究中使用的一项重要技术。DH 技术是开发纯合系的快速方法，可用于加速作物改良计划。单倍体技术也可用于诱变、转化以及基因组学、生物化学和生理学研究等基础研究。由于不同物种以及同一物种内不同基因型的胚胎发生反应存在差异，因此没有一种通用的方案可以在所有物种中产生 DH。在这里，我们描述了在肥皂草（Saponaria vaccaria L.）中开发双单倍体的方法。
