Department of OBG, Rajarajeswari Medical College and Hospital, Bangalore, India.
Department of Molecular Medicine, Rajarajeswari Medical College and Hospital, Bangalore, India.
Pregnancy Hypertens. 2021 Aug;25:225-229. doi: 10.1016/j.preghy.2021.06.004. Epub 2021 Jun 9.
To demonstrate the use of urine congophilia quantification in the prediction and diagnosis of pre-eclampsia using Congo red dot test.
A prospective cohort study in 378 consecutive pregnant women was conducted. All eligible, consenting women of gestational age between 10 and 34 weeks were enrolled in the study. The presence of urinary misfolded proteins was screened by a simple dot test technique on unsupported nitrocellulose membrane using Congo red dye.
The urinary congophilia was increased in urine from women with pre eclampsia compared to healthy pregnant controls. The mean CRR value of pre eclamptic pregnant women (35.2 ± 9.4%) was five times higher than that of mean CRR value of normotensive pregnant women (6.9 ± 4.7%). The mean gestational age at which Congo red test showed positive was 26.95 ± 2.90 weeks and the time taken from CRD positive to development of PE was 4.92 ± 2.54 weeks of gestation.
In our study, the CRD test was not only effective in predicting pre-eclampsia but was also useful in differentiating between pre-eclampsia and other forms of hypertension, as well as early onset and late onset pre-eclampsia, with positive predictive value of 80.36% and negative predictive value of 92.86.
展示使用刚果红斑点试验对尿液 congophilia 定量在预测和诊断子痫前期中的应用。
对 378 例连续妊娠妇女进行前瞻性队列研究。所有符合条件、同意的 10 至 34 周妊娠妇女均纳入研究。使用刚果红染料在无支持的硝酸纤维素膜上通过简单的斑点试验技术筛选尿液中错误折叠的蛋白质。
与健康孕妇对照组相比，子痫前期孕妇尿液中的 congophilia 增加。子痫前期孕妇的平均 CRR 值（35.2±9.4%）是正常血压孕妇平均 CRR 值（6.9±4.7%）的五倍。刚果红试验阳性的平均孕龄为 26.95±2.90 周，从 CRD 阳性到发生 PE 的时间为 4.92±2.54 周妊娠。
在我们的研究中，CRD 试验不仅有效预测子痫前期，而且有助于区分子痫前期和其他形式的高血压，以及早发型和晚发型子痫前期，阳性预测值为 80.36%，阴性预测值为 92.86%。
