科威特反刍动物轮状病毒 A 感染的横断面研究和基因分型。
Cross-sectional study and genotyping of rotavirus-A infections in ruminants in Kuwait.
机构信息
Early Warning Center for Transboundary Animal Diseases-Gulf Cooperation Council, PAAFR, P.O. box 21422, 1307 Safat,, Rabyia, Farwanyia, Kuwait.
Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt.
出版信息
BMC Vet Res. 2021 Jul 17;17(1):245. doi: 10.1186/s12917-021-02944-4.
BACKGROUND
Group A rotaviruses (RVA) are zoonotic pathogens responsible for acute enteritis in human and neonatal ruminants. This research aimed to determine the prevalence of RVA in ruminants (cattle, sheep, and goats) and investigate the circulating RVA genotypes in these animals in Kuwait. We conducted a cross-sectional study to detect RVA in ruminants, using an immunochromatography test (IC), direct sandwich ELISA test, and real-time RT-PCR (RT-qPCR) assay using fecal samples.
RESULTS
A total of 400 cattle, 334 sheep, and 222 goats were examined. The prevalence of RVA was 5.3, 1.2, and 2.3%, respectively, using IC. The ELISA test detected RVA from 4.3% of cattle, 0.9% of sheep, and 1.8% of goats. There was a significant association between the occurrence of diarrhea and the presence of RVA in bovine fecal samples (p-value = 0.0022), while no statistical association between diarrhea and the presence of RVA in fecal samples of sheep and goats was observed (p-value = 0.7250; p-value = 0.4499, respectively). Twenty-three of the IC-positive samples (17 from cattle, two from sheep, and four from goats) were tested using a RT-qPCR RVA detection assay targeting the NSP3 gene. The results showed that 21 of 23 IC-positive samples tested positive by RT-qPCR. Detection of RVA genotypes revealed that G10P[11] was the predominant strain in cattle (58.8%), followed by G8P[1] (11.7%). One sheep sample was genotyped as G8P[1]. In addition, G6P[1] and G6P[14] were detected in goat samples.
CONCLUSION
The present study revealed that the IC was more sensitive in detecting RVA antigen in fecal samples than the ELISA test. A higher occurrence of RVA infection was observed in cattle than in sheep and goats. This study suggests that RVA might be a risk factor of diarrhea in bovine calves less than 2 weeks old. This research also demonstrates the circulation of RVA in sheep and goat populations in Kuwait. Finally, the G10P[11] RVA genotype was the most prevalent genotype identified from cattle samples.
背景
A 组轮状病毒（RVA）是一种人畜共患病病原体，可导致人类和新生反刍动物急性肠炎。本研究旨在确定 RVA 在反刍动物（牛、绵羊和山羊）中的流行情况，并调查这些动物中循环的 RVA 基因型。我们使用免疫层析试验（IC）、直接夹心 ELISA 试验和粪便样本的实时 RT-PCR（RT-qPCR）检测，对反刍动物进行了横断面研究。
结果
共检查了 400 头牛、334 只绵羊和 222 只山羊。使用 IC，RVA 的流行率分别为 5.3%、1.2%和 2.3%。ELISA 试验检测到 4.3%的牛、0.9%的绵羊和 1.8%的山羊存在 RVA。牛粪便样本中 RVA 的出现与腹泻之间存在显著关联（p 值=0.0022），而绵羊和山羊粪便样本中腹泻与 RVA 存在之间未观察到统计学关联（p 值=0.7250；p 值=0.4499）。用针对 NSP3 基因的 RT-qPCR RVA 检测试验对 23 份 IC 阳性样本（17 份来自牛、2 份来自绵羊和 4 份来自山羊）进行了检测。结果表明，23 份 IC 阳性样本中，有 21 份通过 RT-qPCR 检测呈阳性。检测到的 RVA 基因型显示，G10P[11]是牛中主要流行株（58.8%），其次是 G8P[1]（11.7%）。一个绵羊样本被鉴定为 G8P[1]。此外，在山羊样本中检测到 G6P[1]和 G6P[14]。
结论
本研究表明，IC 比 ELISA 试验更灵敏地检测粪便样本中的 RVA 抗原。牛中 RVA 感染的发生率高于绵羊和山羊。本研究表明，RVA 可能是 2 周龄以下牛犊腹泻的危险因素。本研究还表明，RVA 在科威特绵羊和山羊群体中循环。最后，从牛样本中鉴定出的最常见 RVA 基因型是 G10P[11]。
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