监测荧光标记的固定化和游离假单胞菌 oleovorans 的生长、存活和苯酚利用情况。

Monitoring the growth, survival and phenol utilization of the fluorescent-tagged Pseudomonas oleovorans immobilized and free cells.

机构信息

Director's Research Cell, CSIR-National Environmental Engineering Research Institute, Nagpur 440020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.

Director's Research Cell, CSIR-National Environmental Engineering Research Institute, Nagpur 440020, India.

出版信息

Bioresour Technol. 2021 Oct;338:125568. doi: 10.1016/j.biortech.2021.125568. Epub 2021 Jul 14.

DOI:10.1016/j.biortech.2021.125568

PMID:34274579

Abstract

Bioaugmentation in wastewater treatment plants (WWTPs) is challenging due to low survival and persistence of applied microbes. This study aimed to track the capacity and survival of fluorescent-tagged Pseudomonas oleovoransICTN13 as a model organism applicable in bioaugmentation of phenol-containing wastewater. The isolate was immobilized in alginate biopolymer, and enhanced efficacy and survival for biodegradation of phenol against free cells were studied. Encapsulated cells resulted in enhanced phenol removal efficiency (~~94%) compared to free cells (~~72%). Encapsulation of cells facilitated an extended storage time of 30 days. Remarkably, phenol and COD removal efficacy of encapsulated cells was sustained up to ~ 92-93% in a reactor after 45 days, while free cells could produce ~ 80-84% removal efficiency. Fluorescence microscopy showed high survival of the encapsulated cells, whereas gradual deterioration of free cells was observed. Thus, the findings highlight the importance of bio augmented strain in WWTPs where encapsulation is a crucial factor.

摘要

在废水处理厂（WWTPs）中进行生物增强具有挑战性，因为应用的微生物的存活率和持久性较低。本研究旨在追踪荧光标记的假单胞菌 oleovoransICTN13 的能力和存活情况，该菌作为一种适用于含酚废水生物增强的模型生物。将分离株固定在藻酸盐生物聚合物中，并研究了其对苯酚生物降解的增强效果和游离细胞的生存能力。与游离细胞（约 72%）相比，包封细胞可提高苯酚去除效率（约 94%）。细胞包封有助于延长 30 天的储存时间。值得注意的是，在 45 天后，在反应器中，包封细胞的苯酚和 COD 去除效率可持续保持在约 92-93%，而游离细胞的去除效率约为 80-84%。荧光显微镜显示包封细胞的存活率很高，而游离细胞则逐渐恶化。因此，这些发现强调了在 WWTPs 中使用生物增强菌株的重要性，其中封装是一个关键因素。