Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
Faculty of Natural Sciences, Novosibirsk State University, Novosibirsk 630090, Russia.
Viruses. 2024 Sep 30;16(10):1561. doi: 10.3390/v16101561.
Bacteria of the genus, including the subgroup, play an important role in the environmental microbial communities. Psychrotolerant isolates of can produce thermostable proteases and lipases. When contaminating refrigerated raw milk, these bacteria spoil it by producing enzymes resistant to pasteurization. One possible way to prevent spoilage of raw milk is to use lytic phages specific to undesirable isolates. The first phage, vB_PseuGesM_254, was isolated and characterized, which is active against several proteolytic strains. This lytic myophage can infect and lyse its host strain at 24 °C and at low temperature (8 °C); so, it has the potential to prevent contamination of raw milk. The vB_PseuGesM_254 genome, 95,072 bp, shows a low level of intergenomic similarity with the genomes of known phages. Comparative proteomic ViPTree analysis indicated that vB_PseuGesM_254 is associated with a large group of phages that are members of the and subfamilies and the genus. The alignment constructed using ViPTree shows that the vB_PseuGesM_254 genome has a large inversion between ~53,100 and ~70,700 bp, which is possibly a distinctive feature of a new taxonomic unit within this large group of phages.
包括亚群在内的属细菌在环境微生物群落中发挥着重要作用。可耐受低温的可以产生热稳定的蛋白酶和脂肪酶。当污染冷藏的生牛乳时，这些细菌通过产生对巴氏杀菌有抗性的酶来使牛奶变质。防止生牛乳变质的一种可能方法是使用针对不良分离株的裂解噬菌体。第一个噬菌体 vB_PseuGesM_254 已被分离和鉴定，它对几种蛋白水解菌株具有活性。这种裂解噬菌体可以在 24°C 和低温（8°C）下感染和裂解其宿主菌株；因此，它有可能防止生牛乳受到污染。vB_PseuGesM_254 的基因组为 95,072bp，与已知噬菌体的基因组具有较低的基因组间相似性。比较蛋白质组学 ViPTree 分析表明，vB_PseuGesM_254 与属于 和 亚科以及 属的一大群噬菌体有关。使用 ViPTree 构建的比对表明，vB_PseuGesM_254 基因组在约 53100bp 和 70700bp 之间存在较大的倒位，这可能是该大群噬菌体中新分类单元的一个独特特征。
