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通过全基因组测序分析从奶粉和配方奶中分离出的菌株的毒力和抗生素抗性基因

Profiling the Virulence and Antibiotic Resistance Genes of Strains Isolated From Powdered and Dairy Formulas by Whole-Genome Sequencing.

作者信息

Parra-Flores Julio, Holý Ondrej, Riffo Francisca, Lepuschitz Sarah, Maury-Sintjago Eduard, Rodríguez-Fernández Alejandra, Cruz-Córdova Ariadnna, Xicohtencatl-Cortes Juan, Mancilla-Rojano Jetsi, Troncoso Miriam, Figueroa Guillermo, Ruppitsch Werner, Forsythe Stephen

机构信息

Department of Nutrition and Public Health, Universidad del Bío-Bío, Chillán, Chile.

Department of Public Health, Palacký University Olomouc, Olomouc, Czechia.

出版信息

Front Microbiol. 2021 Jun 30;12:694922. doi: 10.3389/fmicb.2021.694922. eCollection 2021.

DOI:10.3389/fmicb.2021.694922
PMID:34276629
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8278472/
Abstract

is an enteropathogen that causes neonatal meningitis, septicemia, and necrotizing enterocolitis in preterm infants and newborns with a mortality rate of 15 to 80%. Powdered and dairy formulas (P-DF) have been implicated as major transmission vehicles and subsequently the presence of this pathogen in P-DF led to product recalls in Chile in 2017. The objective of this study was to use whole genome sequencing (WGS) and laboratory studies to characterize strains from the contaminated products. Seven strains were identified as , and the remaining strain was . All strains adhered to a neuroblastoma cell line, and 31 virulence genes were predicted by WGS. The antibiograms varied between strains. and included and genes, conferring resistance to colistin and cephalothin, respectively. The strains encoded I-E and I-F CRISPR-Cas systems, and carried IncFII(pECLA), Col440I, and Col(pHHAD28) plasmids. In summary, WGS enabled the identification of strains and revealed multiple antibiotic resistance and virulence genes. These findings support the decision to recall the contaminated powdered and dairy formulas from the Chilean market in 2017.

摘要

是一种肠道病原体，可导致早产儿和新生儿患新生儿脑膜炎、败血症和坏死性小肠结肠炎，死亡率为15%至80%。粉状和乳类配方奶粉（P-DF）被认为是主要传播媒介，随后该病原体在P-DF中的存在导致2017年智利的产品召回。本研究的目的是使用全基因组测序（WGS）和实验室研究来鉴定受污染产品中的菌株特征。鉴定出7株为 ，其余1株为 。所有 菌株均黏附于神经母细胞瘤细胞系，通过WGS预测出31个毒力基因。各菌株的抗菌谱各不相同。 包括 和 基因，分别赋予对黏菌素和头孢噻吩的抗性。 菌株编码I-E和I-F CRISPR-Cas系统，并携带IncFII(pECLA)、Col440I和Col(pHHAD28)质粒。总之，WGS能够鉴定 菌株，并揭示多种抗生素抗性和毒力基因。这些发现支持了2017年从智利市场召回受污染的粉状和乳类配方奶粉的决定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d4/8278472/143a9db9560f/fmicb-12-694922-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d4/8278472/16c0fc654c79/fmicb-12-694922-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d4/8278472/143a9db9560f/fmicb-12-694922-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d4/8278472/16c0fc654c79/fmicb-12-694922-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d4/8278472/143a9db9560f/fmicb-12-694922-g002.jpg

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