Department of Obstetrics and Gynecology, Harbin Red Cross Central Hospital, Harbin, Heilongjiang, China.
Department of Obstetrics, Yihe Maternity District of Cangzhou People's Hospital, Cangzhou, Hebei, China.
Biotechnol Appl Biochem. 2022 Aug;69(4):1509-1516. doi: 10.1002/bab.2223. Epub 2021 Aug 1.
Luteinizing hormone (LH)/lutropin is an interstitial cell-stimulating hormone playing a predominant role in the reproductive system, and highly correlated with the infertility treatment in both men and women. This research was concentrated to quantify LH level by using interdigitated electrode sensor. To improve the electric current flow, sensing electrode was modified with graphene oxide (GO) and the aptamer probe was attached on GO through biotin-streptavidin linker. Current responses were measured with aptamer-LH interaction at the target concentrations between 7.5 nM and 1 μM and the detection limit of LH was calculated as 60 nM with the determination coefficient (R ) value, 0.9229 [y = 1.296x - 2.8435] on a linear range from 30 nM to 1 μM. Further, biofouling effect on sensing electrode surface was analyzed with complementary aptamer sequence, control proteins (albumin and globulin). The above GO-aptamer-modified interdigitated electrode sensor helps to quantify LH level and diagnose gynecological endocrinology-related complications.
黄体生成素（LH）/促黄体激素是一种间质细胞刺激激素，在生殖系统中起着主要作用，与男性和女性的不孕治疗高度相关。本研究集中于使用叉指电极传感器来定量 LH 水平。为了改善电流流动，通过生物素-链霉亲和素连接子将传感电极用氧化石墨烯（GO）进行修饰，并将适配体探针连接到 GO 上。通过在靶浓度为 7.5 nM 至 1 μM 之间的适配体-LH 相互作用测量电流响应，LH 的检测限计算为 60 nM，线性范围为 30 nM 至 1 μM 时的决定系数（R）值为 0.9229 [y = 1.296x - 2.8435]。此外，还使用互补适配体序列、对照蛋白（白蛋白和球蛋白）分析了传感电极表面的生物污垢效应。上述 GO-适配体修饰的叉指电极传感器有助于定量 LH 水平并诊断妇科内分泌相关并发症。
