Department of General Surgery III, Xinxiang Central Hospital, The Fourth Clinical College of Xinxiang Medical University, Xinxiang City, Henan Province, 453000, China.
Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis, Arau 02600, Perlis, Malaysia.
J Biomed Nanotechnol. 2021 Dec 1;17(12):2495-2504. doi: 10.1166/jbn.2021.3213.
An aptasensor was developed on an interdigitated microelectrode (IDME) by current-volt sensing for the diagnosis of ulcerative colitis by detecting the biomarker lipocalin-2. Higher immobilization of the anti-lipocalin-2 aptamer as a probe was achieved by using sodium dodecyl benzenesulfonate-aided zeolite particles. FESEM and FETEM observations revealed that the size of the zeolite particles was <200 nm, and they displayed a uniform distribution and spherical shape. XPS analysis attested the occurrence of Si, Al, and O groups on the zeolite particles. Zeolite particles were immobilized on IDME by a (3-aminopropyl)-trimethoxysilane amine linker, and then, the aptamer as the probe was tethered on the zeolite particles through a biotin-streptavidin strategy assisted by a bifunctional aldehyde linker. Due to the high occupancy of the aptamer and the efficient electric transfer from zeolite particles, higher changes in current can be observed upon interaction of the aptamer with lipocalin-2. The lower detection of lipocalin-2 was noted as 10 pg/mL, with a linear range from 10 pg/mL to 1 g/mL and a linear regression equation of y=8E-07x+8E-08; R² = 0.991. Control experiments with complementary aptamer and matrix metalloproteinase-9 indicate the specific detection of lipocalin-2. Furthermore, spiking lipocalin-2 in human serum does not interfere with the identification.
基于电流-电压传感，在叉指微电极（IDME）上开发了适体传感器，通过检测生物标志物脂联素-2 来诊断溃疡性结肠炎。通过使用十二烷基苯磺酸钠辅助沸石颗粒，实现了更高的抗脂联素-2 适体作为探针的固定化。FESEM 和 FETEM 观察表明，沸石颗粒的尺寸<200nm，它们呈现均匀的分布和球形。XPS 分析证明了沸石颗粒上存在 Si、Al 和 O 基团。通过（3-氨丙基）-三甲氧基硅烷胺接头将沸石颗粒固定在 IDME 上，然后通过双功能醛接头辅助的生物素-链霉亲和素策略将适体作为探针连接到沸石颗粒上。由于适体的高占有率和沸石颗粒的有效电子转移，当适体与脂联素-2 相互作用时，可以观察到更高的电流变化。脂联素-2 的检测下限为 10pg/mL，线性范围为 10pg/mL 至 1g/mL，线性回归方程为 y=8E-07x+8E-08；R²=0.991。与互补适体和基质金属蛋白酶-9 的对照实验表明，该传感器可以特异性检测脂联素-2。此外，在人血清中添加脂联素-2 不会干扰鉴定。
