Department of Zoology and Entomology, University of Pretoria, Hatfield, Pretoria, South Africa.
Department of Veterinary Services, Tsetse and Trypanosomiasis Control Unit, Ministry of Fisheries and Livestock, Lusaka, Zambia.
PLoS Negl Trop Dis. 2021 Jul 19;15(7):e0009620. doi: 10.1371/journal.pntd.0009620. eCollection 2021 Jul.
Black screen fly round (BFR) is a mobile sampling method for Glossina morsitans. This technique relies on the ability of operator(s) to capture flies landing on the screen with hand nets. In this study, we aimed to evaluate a vehicle-mounted sticky panel trap (VST) that is independent of the operator's ability to capture flies against BFR, for effective and rapid sampling of G. m. morsitans Westwood and G. m. centralis Machado. We also determined the influence of the VST colour (all-blue, all-black or 1:1 blue-black), orientation and presence of odour attractants on tsetse catch.
METHODOLOGY/PRINCIPAL FINDINGS: Using randomised block design experiments conducted in Zambia, we compared and modelled the number of tsetse flies caught in the treatment arms using negative binomial regression. There were no significant differences in the catch indices of the three colour designs and for in-line or transversely oriented panels for both subspecies (P > 0.05). When baited with butanone and 1-octen-3-ol, VST caught 1.38 (1.11-1.72; P < 0.01) times more G. m. centralis flies than the un-baited trap. Attractants did not significantly increase the VST catch index for G. m. morsitans (P > 0.05). Overall, the VST caught 2.42 (1.91-3.10; P < 0.001) and 2.60 (1.50-3.21; P < 0.001) times more G. m. centralis and G. m. morsitans respectively, than the BFR. The VST and BFR took 10 and 35 min respectively to cover a 1 km transect.
CONCLUSION/SIGNIFICANCE: The VST is several times more effective for sampling G. m. morsitans and G. m. centralis than the BFR and we recommend its use as an alternative sampling tool.
黑幕蝇旋飞（BFR）是一种用于采捕舌蝇的移动采样方法。这种技术依赖于操作者用手网捕捉落在屏幕上的苍蝇的能力。在这项研究中，我们旨在评估一种独立于操作者捕捉苍蝇能力的车载粘性面板诱捕器（VST），以便有效地快速采集 Glossina morsitans Westwood 和 Glossina m. centralis Machado。我们还确定了 VST 颜色（全蓝、全黑或 1:1 蓝黑）、方向和气味引诱剂的存在对采蝇的影响。
方法/主要发现：我们在赞比亚进行了随机分组设计实验，使用负二项回归比较和模拟处理组中采蝇的数量。三种颜色设计的捕获指数以及对于两种亚种的纵向或横向定向面板之间没有显著差异（P > 0.05）。当用丁酮和 1-辛烯-3-醇引诱时，VST 捕获的 G. m. centralis 苍蝇比未引诱的诱捕器多 1.38 倍（1.11-1.72；P < 0.01）。引诱剂并没有显著增加 G. m. morsitans 的 VST 捕获指数（P > 0.05）。总的来说，VST 捕获的 G. m. centralis 和 G. m. morsitans 苍蝇分别比 BFR 多 2.42 倍（1.91-3.10；P < 0.001）和 2.60 倍（1.50-3.21；P < 0.001）。VST 和 BFR 分别需要 10 分钟和 35 分钟才能覆盖 1 公里的样带。
结论/意义：VST 比 BFR 更有效地采集 G. m. morsitans 和 G. m. centralis，我们建议将其作为替代采样工具使用。
