Ross University School of Veterinary Medicine, 42123 Basseterre, Saint Kitts and Nevis.
Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
Int J Mol Sci. 2021 Jul 1;22(13):7128. doi: 10.3390/ijms22137128.
is a bacterial pathogen mostly of ruminant livestock in the tropics/subtropics and certain temperate climate areas. It causes dermatophilosis, a skin disease that threatens food security by lowering animal productivity and compromising animal health and welfare. Since it is a prevalent infection in ruminants, dermatophilosis warrants more research. There is limited understanding of its pathogenicity, and as such, there is no registered vaccine against To better understanding the genomics of , the primary aim of this work was to investigate this bacterium using whole-genome sequencing and bioinformatic analysis. is a high GC member of the Actinobacteria and encodes approximately 2527 genes. It has an open pan-genome, contains many potential virulence factors, secondary metabolites and encodes at least 23 housekeeping genes associated with antimicrobial susceptibility mechanisms and some isolates have an acquired antimicrobial resistance gene. Our isolates contain a single CRISPR array Cas type IE with classical 8 Cas genes. Although the isolates originate from the same geographical location there is some genomic diversity among them. In conclusion, we present the first detailed genomic study on , including the first observation of (Z), a tetracycline resistance-conferring gene.
是一种主要存在于热带/亚热带和某些温带气候地区反刍家畜中的细菌病原体。它会引起皮肤坏死梭菌病，这是一种皮肤疾病，会降低动物的生产力，损害动物的健康和福利，从而威胁到粮食安全。由于它是反刍动物中的一种普遍感染，因此需要对皮肤坏死梭菌病进行更多的研究。人们对其致病性的了解有限，因此目前尚无针对该菌的注册疫苗。为了更好地了解皮肤坏死梭菌的基因组，这项工作的主要目的是使用全基因组测序和生物信息学分析来研究这种细菌。 是放线菌中的一个高 GC 成员，约编码 2527 个基因。它具有一个开放的泛基因组，包含许多潜在的毒力因子、次生代谢物，并且至少编码 23 个与抗菌药物敏感性机制相关的管家基因，一些分离株还具有获得性抗菌药物耐药基因。我们的分离株含有一个单一的 CRISPR 数组 Cas 类型 IE 和经典的 8 个 Cas 基因。尽管这些分离株起源于同一地理位置，但它们之间存在一些基因组多样性。总之，我们首次对皮肤坏死梭菌进行了详细的基因组研究，包括首次观察到（Z），这是一种赋予四环素耐药性的基因。
