Paz-Ares J, Ghosal D, Wienand U, Peterson P A, Saedler H
Max-Planck Institut für Züchtungsforschung, Köln, FRG.
EMBO J. 1987 Dec 1;6(12):3553-8. doi: 10.1002/j.1460-2075.1987.tb02684.x.
The structure of the wild-type c1 locus of Zea mays was determined by sequence analysis of one genomic and two cDNA clones. The coding region is composed of three exons (150 bp, 129 bp and one, at least 720 bp) and two small introns (88 bp and 145 bp). Transcription of the mRNAs corresponding to the two cDNA clones cLC6 (1.1 kb) and cLC28 (2.1 kb) starts from the same promoter. Both cDNAs are identical except that cLC28 extends further at its 3' end. A putative protein, 273 amino acids in length was deduced from the sequence of both transcripts. It contains two domains, one basic and the other acidic and might function as a transcriptional activator. The basic domain of this c1-encoded protein shows 40% sequence homology to the protein products of animal myb proto-oncogenes.
通过对一个基因组克隆和两个cDNA克隆进行序列分析，确定了玉米野生型c1基因座的结构。编码区由三个外显子（150 bp、129 bp和至少720 bp的一个）和两个小内含子（88 bp和145 bp）组成。与两个cDNA克隆cLC6（1.1 kb）和cLC28（2.1 kb）相对应的mRNA转录起始于同一个启动子。除了cLC28在其3'端延伸得更远之外，两个cDNA是相同的。从两个转录本的序列推导出一个长度为273个氨基酸的推定蛋白质。它包含两个结构域，一个碱性结构域和另一个酸性结构域，可能作为转录激活因子发挥作用。这种由c1编码的蛋白质的碱性结构域与动物myb原癌基因的蛋白质产物具有40%的序列同源性。
