Wang Tao, Zhao Shuo, Wang Bo, Li Jianan, Ye Zengrong, Zhang Famei, Ma Huiyuan, Zhou Guoying
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, China.
University of Chinese Academy of Sciences, Beijing, China.
Front Plant Sci. 2025 May 16;16:1558321. doi: 10.3389/fpls.2025.1558321. eCollection 2025.
( Maxim. ex Regel) is a herbaceous plant belonging to Polygonaceae family and L. genus. It holds considerable value in culinary and medicinal realms, primarily due to their rich Anthraquinones (AQs) content. Understanding the molecular mechanisms that regulate AQs biosynthesis is a prerequisite for increasing their yield. MYB transcription factors (TFs) can regulate the synthesis of a variety of plant secondary metabolites. However, only a few research have explored the role of MYB TFs in L. species. In this study, 1054 MYB genes from four L. species were identified. The number of MYB genes in each species was similar, distributed across 11 chromosomes. To investigate the phylogeny of identified MYB TFs, they were classified into four subfamilies. Sequence characteristics, phylogenetic relationships, evolutionary trends, and tissue expression of MYB genes in L. species were further studied. Subsequently, 12 MYB genes were selected, which shown differential expression in different tissues. Further research on these genes indicated a significant correlation with genes in shikimate pathway and polyketide pathway of AQs biosynthesis. Protein-protein interaction simulations in and qRT-PCR experiments further confirmed this situation. This research lays the foundation for studying molecular mechanisms by which MYB TFs regulates AQs biosynthesis in four L. species.
（大黄属某植物）是一种属于蓼科大黄属的草本植物。它在烹饪和医药领域具有相当大的价值，主要是因为其富含蒽醌类化合物（AQs）。了解调节AQs生物合成的分子机制是提高其产量的前提条件。MYB转录因子（TFs）可以调节多种植物次生代谢产物的合成。然而，只有少数研究探索了MYB TFs在大黄属植物中的作用。在本研究中，从四种大黄属植物中鉴定出1054个MYB基因。每个物种中MYB基因的数量相似，分布在11条染色体上。为了研究已鉴定的MYB TFs的系统发育，它们被分为四个亚科。进一步研究了大黄属植物中MYB基因的序列特征、系统发育关系、进化趋势和组织表达。随后，选择了12个在不同组织中表现出差异表达的MYB基因。对这些基因的进一步研究表明，它们与AQs生物合成的莽草酸途径和聚酮途径中的基因存在显著相关性。在（某种植物）中的蛋白质-蛋白质相互作用模拟和qRT-PCR实验进一步证实了这种情况。本研究为研究MYB TFs调节四种大黄属植物中AQs生物合成的分子机制奠定了基础。
