Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
J Agric Food Chem. 2021 Aug 4;69(30):8492-8503. doi: 10.1021/acs.jafc.1c02759. Epub 2021 Jul 20.
(-)-α-Bisabolol is a functional ingredient in various health and cosmetic products and has antibacterial, anti-inflammatory, and wound healing properties. (-)-α-Bisabolol is chemically synthesized and produced by steam distillation of essential oils extracted from Brazilian Candeia (). To sustainably produce pure (-)-α-bisabolol, we previously engineered to produce 9.1 g/L (-)-α-bisabolol via heterologous mevalonate pathways and (-)-α-bisabolol synthase (BOS) from German chamomile, (MrBOS). BOS has only been reported in MrBOS and Brazilian Candeia (EeBOS). The limited availability of BOS has made it difficult to achieve high titer and yield and large-scale (-)-α-bisabolol production. We identified a novel BOS in globe artichoke (CcBOS) and examined its functionality and . CcBOS showed higher catalytic efficiency and (-)-α-bisabolol production rates than those from MrBOS or EeBOS. In fed-batch fermentation, CcBOS generated the highest reported (-)-α-bisabolol titer to date (23.4 g/L). These results may facilitate economically viable industrial (-)-α-bisabolol production.
(-)-α- 菠醇是各种健康和美容产品中的一种功能性成分，具有抗菌、抗炎和伤口愈合的特性。(-)-α- 菠醇通过化学合成和巴西坎迪亚（）精油的水蒸气蒸馏生产。为了可持续地生产纯(-)-α- 菠醇，我们之前通过异源甲羟戊酸途径和来自德国甘菊的（MrBOS）(-)-α- 菠醇合酶（BOS）工程化 来生产 9.1 g/L 的(-)-α- 菠醇。BOS 仅在 MrBOS 和巴西坎迪亚（EeBOS）中报道过。BOS 的供应有限，使得难以实现高滴度和产量以及大规模(-)-α- 菠醇生产。我们在朝鲜蓟（CcBOS）中鉴定出一种新型 BOS，并研究了其功能 。CcBOS 显示出比 MrBOS 或 EeBOS 更高的催化效率和(-)-α- 菠醇产率。在分批补料发酵中，CcBOS 产生了迄今为止报道的最高(-)-α- 菠醇滴度（23.4 g/L）。这些结果可能有助于经济可行的工业(-)-α- 菠醇生产。
