Reid D M, Loeffler K U, Campbell A M, Forrester J V
Department of Biochemistry, University of Glasgow, U.K.
Exp Eye Res. 1987 Nov;45(5):731-45. doi: 10.1016/s0014-4835(87)80121-5.
This report describes the ultrastructural localization of S-antigen in pig and human retinas by means of a highly specific rat IgG monoclonal antibody, S2.4.C5, followed by a secondary antibody adsorbed to colloidal gold. This monoclonal antibody gave definitive staining with negligible background. The protein was detected in both the rod outer and inner segments. Connecting cilia and the rod outer segment disc membranes were labelled. The outer segment plasma membrane was not obviously labelled. Cones were labelled at background level. S-Antigen was not detected in any other cells of the neural retina. The fate of S-antigen was also followed to the pigment epithelial phagosomes where intracytoplasmic ROS debris was stained with the antibody. No label, however, was detected in the choroid, suggesting that trans-cellular transport of the S-antigen did not occur.
本报告描述了通过一种高度特异性的大鼠IgG单克隆抗体S2.4.C5，随后用吸附于胶体金的二抗，在猪和人类视网膜中对S抗原进行超微结构定位。这种单克隆抗体产生了明确的染色，背景可忽略不计。在视杆细胞的外段和内段均检测到了该蛋白。连接纤毛和视杆细胞外段盘膜被标记。视杆细胞外段质膜未被明显标记。视锥细胞的标记处于背景水平。在神经视网膜的任何其他细胞中均未检测到S抗原。S抗原的去向也追踪到了色素上皮吞噬体，其中胞浆内的ROS碎片被该抗体染色。然而，在脉络膜中未检测到标记，这表明S抗原没有发生跨细胞转运。
