Zeimer R C, Lim H K, Ogura Y
Department of Ophthalmology, University of Illinois College of Medicine, Chicago 60612.
Exp Eye Res. 1987 Dec;45(6):969-76. doi: 10.1016/s0014-4835(87)80110-0.
Because the results of various vision tests and diagnostic methods are influenced by the amount of light transmitted by the lens, it would be useful to quantify changes in transmittance. We have developed a new method capable of following these changes objectively and quantitatively. The method is based on an increasing loss of fluorescence emitted by the lens substance as the probing volume is located more posteriorly along the lens axis. The loss of fluorescence is quantified by a parameter called the fluorescence reading ratio (FRR). In the present study, performed on postmortem human lenses, we evaluated the validity of our proposed in vivo method by comparing it with measurements of lens transmittance by spectrophotometry. The correlation of FRR with the transmittance between 420- and 500 nm (r greater than 0.7, P less than 0.015) indicates that the measurement of FRR can provide information on the transmittance at these wavelengths. In addition, a correlation was found between age and transmittance at 440 nm (r = 0.93, P less than 0.001) and FRR (r = 0.88, P less than 0.001).
由于各种视力测试和诊断方法的结果会受到晶状体透光量的影响，因此对透光率的变化进行量化将很有帮助。我们开发了一种能够客观且定量地跟踪这些变化的新方法。该方法基于随着探测体积沿晶状体轴更向后定位，晶状体物质发出的荧光损失增加。荧光损失通过一个称为荧光读数比（FRR）的参数进行量化。在对死后人类晶状体进行的本研究中，我们通过将其与分光光度法测量的晶状体透光率进行比较，评估了我们提出的体内方法的有效性。FRR与420至500纳米之间的透光率的相关性（r大于0.7，P小于0.015）表明，FRR的测量可以提供这些波长下透光率的信息。此外，在440纳米处的透光率与年龄之间（r = 0.93，P小于0.001）以及FRR与年龄之间（r = 0.88，P小于0.001）发现了相关性。
