哺乳动物 I、II 和 III 型味觉细胞标志物在鸡味蕾中的重叠分布。
Overlapping distributions of mammalian types I, II, and III taste cell markers in chicken taste buds.
机构信息
Department of Food and Life Sciences, College of Agriculture, Ibaraki University, Ami, Japan.
Physiology of Domestic Animals, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Japan.
出版信息
Biochem Biophys Res Commun. 2021 Sep 17;570:162-168. doi: 10.1016/j.bbrc.2021.07.025. Epub 2021 Jul 17.
Mammalian taste buds comprise types I, II, and III taste cells, with each type having specific characteristics: glia-like supporting cells (type I), taste receptor cells (type II), and presynaptic cells (type III). In this study, to characterize the peripheral taste-sensing systems in chickens, we analyzed the distributions of the mammalian types I, II, and III taste cell markers in chicken taste buds: glutamate-aspartate transporter (GLAST) for type I; taste receptor type 1 members 1 and 3 (T1R1 and T1R3), taste receptor type 2 member 7 (T2R7), and α-gustducin for type II; and synaptosomal protein 25 (SNAP25) and neural cell adhesion molecule (NCAM) for type III. We found that most GLAST taste cells expressed α-gustducin and SNAP25 and that high percentages of T1R3 or α-gustducin taste cells expressed SNAP25 and NCAM. These results demonstrated a unique subset of chicken taste cells expressing multiple taste cell type marker proteins. Taken together, these results provide new insights into the taste-sensing mechanisms in vertebrate taste buds.
哺乳动物的味蕾包括 I 型、II 型和 III 型味觉细胞，每种类型都具有特定的特征：胶质样支持细胞（I 型）、味觉受体细胞（II 型）和突触前细胞（III 型）。在这项研究中，为了表征鸡的外周味觉感应系统，我们分析了鸡味蕾中哺乳动物 I 型、II 型和 III 型味觉细胞标记物的分布：谷氨酸-天冬氨酸转运体（GLAST）用于 I 型；味觉受体 1 型成员 1 和 3（T1R1 和 T1R3）、味觉受体 2 型成员 7（T2R7）和 α- gustducin 用于 II 型；和突触体蛋白 25（SNAP25）和神经细胞粘附分子（NCAM）用于 III 型。我们发现大多数 GLAST 味觉细胞表达 α- gustducin 和 SNAP25，并且高比例的 T1R3 或 α- gustducin 味觉细胞表达 SNAP25 和 NCAM。这些结果表明鸡味觉细胞表达多种味觉细胞类型标记蛋白的独特亚群。总之，这些结果为脊椎动物味蕾的味觉感应机制提供了新的见解。
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