A novel synthesis of a chlorophyll b-gold nanoconjugate used for enhancing photodynamic therapy: In vitro study.

Chlorophyll, the essential green pigment in plants, is considered a promising natural photosensitizer (PS) for photodynamic therapy (PDT). However, it suffers from lower stability in the physiological conditions that depress its efficacy in the PDT. The combination of nanotechnology and PDT is becoming a promising approach to combat tumors. Gold nanoparticles (Au NPs), for example, are proposed as suitable carriers that can increase chlorophyll stability when conjugating together. In the present work, the impact of Au NPs conjugation in enhancing Chlorophyll b (Chl b) efficiency in the PDT of cancer cells has been emphasized. A chemical method using a natural product synthesized a novel Chlorophyll b-gold nanoparticles nanoconjugate (Chl b-Au NCs). The synthesized Chl b-Au NCs were characterized via UV-Vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), Laser-Induced Fluorescence (LIF), Zeta potential, Dynamic light scattering (DLS), and Transmission electron microscopy (TEM). Chl b is characterized by a formyl group (CHO) which is absent in Chl a. This group leads to the formation of an electrostatic reaction between the positive charge of Chl b and the negative charge present on the surface of the gold nanoparticles. Moreover, Chlorophyll b loading on the biosynthesized gold nanoparticles (Au NPs) increases its photostability. The efficiency of the PDT was then studied on the MCF7 and the HepG2 cells using this conjugation. As a result, the prepared Chl b-Au NCs showed low dark toxicity, excellent photostability under laser irradiation of wavelength 650 nm, in addition to a significantly high PDT efficacy against tumor cells in vitro. This is due to the enhanced cellular uptake and the high reactive oxygen species (ROS) production upon laser irradiation. Therefore, the designed Chl b-Au NCs could be a photo-therapeutic agent for enhancing cancer therapy in future applications.