Alexeree Shaimaa M I, Sliem Mahmoud A, El-Balshy Ragaa M, Amin Rehab M, Harith M A
National Institute of Laser Enhanced Science, Cairo University, Egypt.
Department of Zoology, Faculty of Science, Benha University, Egypt.
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:727-734. doi: 10.1016/j.msec.2017.03.129. Epub 2017 Mar 18.
Increasing the limit of dispersion of metal-free phthalocyanine (HPc) in an aqueous medium using biosynthetic gold nanoparticles for photodynamic therapy (PDT) is investigated. Gold nanoparticles (Au NPs) are biosynthesized in one step using Potatoes (Solanum tuberosum) extract and are characterized by UV/VIS spectrophotometry, Fourier transformer infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The metal-free phthalocyanine is conjugated to the surface of the gold nanoparticles in a side to side regime through the secondary amine groups of HPc. The clear violet solution of phthalocyanine-gold (Pc-Au) nanoconjugates is investigated by UV-VIS, FTIR and TEM techniques. Disappearance of the absorption band of the secondary amine in the Pc-Au nanoconjugates compared to that of the parent HPc, and detection of the absorption band of HPc in the aqueous medium confirmed the dispersion of HPc and consequently the loading of HPc on the surface of Au NPs. The cytotoxic effect of biosynthetic gold nanoparticles and Pc-Au nanoconjugates compared to chemically synthesized gold nanoparticles on buffalo epithelial cells has been studied in vitro. Interestingly, the results showed that the biosynthetic Au NPs as well as Pc-Au nanoconjugates have no effect on buffalo epithelial cells viability, which indicating their biocompatibility contrary to the chemically synthesized Au NPs. This work will open the door, for the first time, for using HPc suspended in water for PDT and other phototherapeutic applications.
研究了使用生物合成金纳米颗粒提高无金属酞菁（HPc）在水介质中的分散极限以用于光动力疗法（PDT）。使用马铃薯（Solanum tuberosum）提取物一步生物合成金纳米颗粒（Au NPs），并通过紫外/可见分光光度法、傅里叶变换红外光谱（FTIR）和透射电子显微镜（TEM）对其进行表征。无金属酞菁通过HPc的仲胺基团以并排方式共轭到金纳米颗粒表面。通过紫外-可见光谱、FTIR和TEM技术研究了酞菁-金（Pc-Au）纳米共轭物的澄清紫色溶液。与母体HPc相比，Pc-Au纳米共轭物中仲胺吸收带的消失以及在水介质中检测到HPc的吸收带，证实了HPc的分散，从而证实了HPc负载在Au NPs表面。体外研究了生物合成金纳米颗粒和Pc-Au纳米共轭物与化学合成金纳米颗粒相比对水牛上皮细胞的细胞毒性作用。有趣的是，结果表明生物合成的Au NPs以及Pc-Au纳米共轭物对水牛上皮细胞活力没有影响，这表明它们与化学合成的Au NPs相反具有生物相容性。这项工作将首次为将悬浮在水中的HPc用于PDT和其他光治疗应用打开大门。
