Department of Neurosurgery, Shengli Oilfield Central Hospital, Dongying, Shandong, P.R. China.
Department of Neurosurgery, Weifang Yidu Central Hospital, Qingzhou, Shandong, P.R. China.
Folia Neuropathol. 2021;59(2):174-188. doi: 10.5114/fn.2021.107115.
Glioma is one of the common types of intracranial malignancies, which seriously threatens human health. Circular RNAs (circRNAs) play critical roles in various tumours. This study aims to observe the functions and mechanism of circ_0001017 in glioma progression.
Hsa_circ_0001017 expression was analysed in glioma tissue and cells using qRT-PCR assay. Flow cytometer, Cell Counting Kit-8 (CCK-8), colony formation, wound healing, and Transwell were used to analyse glioma cell apoptosis, viability, colony-forming ability, migration, and invasion. The expression of N-deacetylase and N-sulfotransferase 3 (NDST3) and epithelial-mesenchymal transition (EMT)-related proteins was measured using western blot analysis. Luciferase reporter and RNA immunoprecipitation (RIP) assay were performed to determine the target relationship.
In glioma tissues and cells, hsa_circ_0001017 expression was decreased. The overexpression of hsa_circ_0001017 inhibited glioma cell proliferation, EMT, migration, and invasion and promoted glioma cell apoptosis, while the knockdown of hsa_circ_0001017 caused the opposite results. Mechanistically, hsa_circ_0001017 sponged hsa-let-7g-3p and NDST3 was the target gene of hsa-let-7g-3p. Moreover, the tumour-suppressive role of circ_0001017 was associated with hsa-let-7g-3p and NDST3.
Hsa_circ_0001017 suppressed the growth and metastasis and increased cell apoptosis of glioma, and this effect was associated with hsa-let-7g-3p/NDST3 axis.
神经胶质瘤是颅内常见的恶性肿瘤之一，严重威胁人类健康。环状 RNA（circRNAs）在各种肿瘤中发挥着关键作用。本研究旨在观察 circ_0001017 在神经胶质瘤进展中的作用和机制。
采用 qRT-PCR 法检测神经胶质瘤组织和细胞中 hsa_circ_0001017 的表达。流式细胞术、细胞计数试剂盒-8（CCK-8）、集落形成实验、划痕愈合实验和 Transwell 实验用于分析神经胶质瘤细胞凋亡、活力、集落形成能力、迁移和侵袭。采用 Western blot 分析检测 N-脱乙酰基酶和 N-磺基转移酶 3（NDST3）和上皮间质转化（EMT）相关蛋白的表达。通过荧光素酶报告基因和 RNA 免疫沉淀（RIP）实验确定靶标关系。
在神经胶质瘤组织和细胞中，hsa_circ_0001017 的表达降低。hsa_circ_0001017 的过表达抑制神经胶质瘤细胞增殖、EMT、迁移和侵袭，促进神经胶质瘤细胞凋亡，而 hsa_circ_0001017 的敲低则产生相反的结果。机制上，hsa_circ_0001017 作为 hsa-let-7g-3p 的海绵体，NDST3 是 hsa-let-7g-3p 的靶基因。此外，circ_0001017 的肿瘤抑制作用与 hsa-let-7g-3p 和 NDST3 有关。
hsa_circ_0001017 抑制神经胶质瘤的生长和转移，增加细胞凋亡，其作用与 hsa-let-7g-3p/NDST3 轴有关。
