Ogawa Takako, Sonoike Kintake
School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo, Japan.
Faclty of Education and Integrated Arts and Sciences, Waseda University, Shinjuku-ku, Tokyo, Japan.
Bio Protoc. 2018 May 5;8(9):e2834. doi: 10.21769/BioProtoc.2834.
Chlorophyll fluorescence measurements have been widely used to monitor the condition of photosynthesis. Furthermore, chlorophyll fluorescence from cyanobacteria reflects the condition of respiration, since cyanobacterial photosynthesis shares several components of electron transport chain with respiration. This protocol presents the method to monitor the condition of both photosynthesis and respiration in cyanobacteria simply by measuring chlorophyll fluorescence in the dark and in the light with pulse amplitude modulation (PAM) chlorophyll fluorometer.
叶绿素荧光测量已被广泛用于监测光合作用的状况。此外,蓝细菌的叶绿素荧光反映了呼吸作用的状况,因为蓝细菌的光合作用与呼吸作用共享电子传递链的几个组成部分。本方案介绍了一种方法,即通过使用脉冲幅度调制(PAM)叶绿素荧光仪在黑暗和光照条件下测量叶绿素荧光,来简单地监测蓝细菌中光合作用和呼吸作用的状况。