分泌蛋白信号肽的功能评估
Functional Evaluation of the Signal Peptides of Secreted Proteins.
作者信息
Yin Weixiao, Wang Yufu, Chen Tao, Lin Yang, Luo Chaoxi
机构信息
College of Plant Science and Technology, Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, and Key Lab of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, China.
出版信息
Bio Protoc. 2018 May 5;8(9):e2839. doi: 10.21769/BioProtoc.2839.
Abstract
Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily.
摘要
在此，我们描述了一种可用于评估预测信号肽功能的方法。该方法利用酵母YTK12菌株和pSUC2载体，其中将带有融合预测信号肽的pSUC2载体转化到酵母中。信号肽的功能可通过使用不同的选择培养基和显色反应来评估。在本方案中，我们提供了详细的操作说明以便于实施。
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