Mao Mao, Liang Hongtao, He Jiankang, Kasimu Ayiguli, Zhang Yanning, Wang Ling, Li Xiao, Li Dichen
State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an Jiaotong University, Xi'an 710049, China.
Int J Bioprint. 2021 Jun 4;7(3):362. doi: 10.18063/ijb.v7i3.362. eCollection 2021.
Recapitulating the vascular networks that maintain the delivery of nutrition, oxygen, and byproducts for the living cells within the three-dimensional (3D) tissue constructs is a challenging issue in the tissue-engineering area. Here, a novel coaxial electrohydrodynamic (EHD) bioprinting strategy is presented to fabricate thick pre-vascularized cell-laden constructs. The alginate and collagen/calcium chloride solution were utilized as the outer-layer and inner-layer bioink, respectively, in the coaxial printing nozzle to produce the core-sheath hydrogel filaments. The effect of process parameters (the feeding rate of alginate and collagen and the moving speed of the printing stage) on the size of core and sheath lines within the printed filaments was investigated. The core-sheath filaments were printed in the predefined pattern to fabricate lattice hydrogel with perfusable lumen structures. Endothelialized lumen structures were fabricated by culturing the core-sheath filaments with endothelial cells laden in the core collagen hydrogel. Multilayer core-sheath filaments were successfully printed into 3D porous hydrogel constructs with a thickness of more than 3 mm. Finally, 3D pre-vascularized cardiac constructs were successfully generated, indicating the efficacy of our strategy to engineer living tissues with complex vascular structures.
在三维（3D）组织构建体中重现维持活细胞营养、氧气和代谢产物输送的血管网络是组织工程领域一个具有挑战性的问题。在此，提出了一种新型的同轴电流体动力学（EHD）生物打印策略，以制造厚的预血管化载细胞构建体。在同轴打印喷嘴中，分别将藻酸盐和胶原蛋白/氯化钙溶液用作外层和内层生物墨水，以生产核壳水凝胶细丝。研究了工艺参数（藻酸盐和胶原蛋白的进料速率以及打印台的移动速度）对打印细丝内核线和鞘线尺寸的影响。将核壳细丝按预定义图案打印，以制造具有可灌注管腔结构的格子水凝胶。通过用负载在内核胶原蛋白水凝胶中的内皮细胞培养核壳细丝来制造内皮化管腔结构。多层核壳细丝成功打印成厚度超过3毫米的3D多孔水凝胶构建体。最后，成功生成了3D预血管化心脏构建体，表明我们构建具有复杂血管结构的活组织策略的有效性。
